LOCUS       sequence-57261-e        1255 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1255)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HSPA1LA
FEATURES             Location/Qualifiers
     source          1..1255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggctactgc caagggaatc gccataggca tcgacctggg caccacctac
      121 tcctgtgtgg gggtgttcca gcacggcaag gtggagatca tcgccaacga ccagggcaac
      181 cgcaccaccc ccagctacgt ggccttcaca gacaccgagc ggctcattgg ggatgcggcc
      241 aagaaccagg tagcaatgaa tccccagaac actgtttttg atgctaaacg tctgatcggc
      301 aggaaattta atgatcctgt tgtacaagca gatatgaaac tttggccttt tcaagtgatt
      361 aatgaaggag gcaagcccaa agtccttgtg tcctacaaag gggagaataa agctttctac
      421 cctgaggaaa tctcttcgat ggtattgact aagttgaagg agactgctga ggcctttttg
      481 ggccaccctg tcaccaatgc agtgattacc gtgccagcct atttcaatga ctctcaacgt
      541 caggctacta aggatgcagg tgtgattgct ggacttaatg tgctaagaat catcaatgag
      601 cccacggctg ctgccattgc ctatggttta gataaaggag gtcaaggaga acgacatgtc
      661 ctgatttttg atctgggtgg aggcacattt gatgtgtcaa ttctgaccat agatgatggg
      721 atttttgagg taaaggccac tgctggggac actcacctgg gtggggagga ctttgacaac
      781 aggcttgtga gccacttcgt ggaggagttc aagaggaaac acaaaaagga catcagccag
      841 aacaagcgag ccgtgaggcg gctgcgcacc gcctgcgaga gggccaagag gaccctgtcg
      901 tccagcaccc aggccaacct agaaattgat tcactttatg aaggcattga cttctataca
      961 tccatcacca gagctcgatt tgaagagttg tgtgcagacc tgtttagggg taccctggag
     1021 cctgtagaaa aagcgcttcg ggatgccaag atggataagg ctaaaatcca tgacattgtt
     1081 ttagtagggg gctccacccg catccccaag gtgcagcggc tgcttcagga ctacttcaat
     1141 ggacgtgatc tcaacaagag catcaaccct gatgaggccg tagcatatgg ggctgcggta
     1201 caagcagcca tcctgatggg ggacaagtga cagtaaaggt ggatacggat ccgaa
//
