LOCUS       sequence-57368-e         780 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Sellers WR, Novitch BG, Miyake S, Heith A, Otterson GA, Kaye FJ, 
            Lassar AB, Kaelin WG
  TITLE     Stable binding to E2F is not required for the retinoblastoma protein
            to activate transcription, promote differentiation, and suppress 
            tumor cell growth.
  JOURNAL   Genes Dev. 1998 Jan 1. 12(1):95-106.
  PUBMED    9420334
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1998 Jan 1. 12(1):95-106."
COMMENT     Sequence Label: 413 pSG5L HA RB
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          65..637
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     645..664
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     692..711
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        692..710
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             744..770
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             744..770
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             750..776
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 aagaggcttt tttggaggcc taggcttttg caaaaagctc cggatcgatc ctgagaactt
       61 cagggtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt
      121 atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca
      181 ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc
      241 tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta
      301 acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact
      361 tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat
      421 tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa
      481 tattcttatt ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt
      541 acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc
      601 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
      661 tgtgctgtct catcattttg gcaaagaatt gtaatacgac tcactatagg gcgaattgtc
      721 gacccgggaa gcttaccatg gcctacccct acgacgtgcc cgactacgcc tccctcggat
//
