LOCUS       sequence-57387-e         858 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-GFP(S65T)-TRP1
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..754
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(71..99)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     694..714
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(833..857)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 agcttcgtac gctgcaggtc gacggatccc cgggttaatt aacagtaaag gagaagaact
       61 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      121 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      181 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcacttatgg
      241 tgttcaatgc ttttcaagat acccagatca tatgaaacgg catgactttt tcaagagtgc
      301 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      361 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      421 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      481 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
      541 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      601 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
      661 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
      721 tgggattaca catggcatgg atgaactata caaatagggc gcgccacttc taaataagcg
      781 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac
      841 aaattttaaa gtgactct
//
