LOCUS       sequence-57399-e         797 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Koreishi M, Yu S, Oda M, Honjo Y, Satoh A
  TITLE     CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking.
  JOURNAL   PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 
            2013 Jan 18.
  PUBMED    23349870
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e54382. doi"; pages: " 
            10.1371/journal.pone.0054382. Epub 2013 Jan 18"
COMMENT     Sequence Label: FLAG-Sec31a S1163A #13#904
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..165
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gccaccatgg actacaaaga ccatgacggt
      121 gattataaag atcatgatat cgattacaag gatgacgatg acaaagaatt caagttaaag
      181 gaagtagatc gtacagccat gcaggcatgg agccctgccc agaatcaccc catttaccta
      241 gcaacaggaa catctgctca gcaattggat gcaacattta gtacgaatgc ttcccttgag
      301 atatttgaat tagacctctc tgatccatcc ttggatatga aatcttgtgc cacattctcc
      361 tcttctcaca ggtaccacaa gttgatttgg gggccttata aaatggattc caaaggagat
      421 gtctctggag ttctgattgc aggtggtgaa aatggaaata ttattctcta tgatccttct
      481 aaaattatag ctggagacaa ggaagttgtg attgcccaga atgacaagca tactggccca
      541 gtgagagcct tggatgtgaa cattttccag actaatctgg tagcttctgg tgctaatgaa
      601 tctgaaatct acatatggga tctaaataat tttgcaaccc caatgacacc aggagccaaa
      661 acacagccgc cagaagatat cagctgcatt gcatggaaca gacaagttca gcatatttta
      721 gcatcagcca gtcccagtgg ccgggccact gtatgggatc ttaggaaaaa tgagccaatc
      781 atcaaagtca gtgacca
//
