LOCUS       sequence-57647-e         932 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to 
            EYFP for optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  TITLE     Cre-dependent expression of halorhodopsin
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-double floxed-eNpHR-EYFP-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..87
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    138..171
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     intron          211..414
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(234..254)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gcaggagagc tgtacaagtt ctgctacgag aacgaggtgt aatgaggcgc gccataactt
       61 cgtataatgt atgctatacg aagttattaa gaggtttcat attgctaata gcagctacaa
      121 tccagctacc attctgcata acttcgtata aagtatccta tacgaagtta ttccggagtc
      181 gactctagag gatccggtac ctcacgacac ctgaaatgga agaaaaaaac tttgaaccac
      241 tgtctgaggc ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaaggagaa
      301 ttacatcaag tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc
      361 catcgcataa aacccctccc cccaacctaa agacgacgta ctccaaaagc tcgagaacta
      421 atcgaggtgc ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga
      481 cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt
      541 cctccatttt gagctccctg cagcagggcc gggaagcggc catctttccg ctcacgcaac
      601 tggtgccgac cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc
      661 aggcaccaga gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc
      721 tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc
      781 ggccccaaaa accgaaatac cagtgtgcag atcttggccc gcatttacaa gactatcttg
      841 ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa
      901 agcagcgaga caggcgcgaa ggtgccacca ga
//
