LOCUS       sequence-57672-e         870 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  APEX targeted to the mitochondrial matrix of mammalian cells. Used 
            for proteomic mapping of mitochondria. This construct has been 
            replaced by APEX2 (construct 49386). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY
  TITLE     Proteomic Mapping of Mitochondria in Living Cells via Spatially 
            Restricted Enzymatic Tagging.
  JOURNAL   Science. 2013 Jan 31.
  PUBMED    23371551
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 31."
COMMENT     Sequence Label: pcDNA3-mito-APEX
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..123
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             124..870
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 atgctggcca cccgcgtgtt cagcctggtg ggcaagcgcg ccatcagcac cagcgtgtgc
       61 gtgcgcgccc acaaggatcc aggcaagccc atccccaacc ccctgctggg cctggacagc
      121 accggaaagt cttacccaac tgtgagtgct gattaccagg acgccgttga gaaggcgaag
      181 aagaagctca gaggcttcat cgctgagaag agatgcgctc ctctaatgct ccgtttggca
      241 ttccactctg ctggaacctt tgacaagggc acgaagaccg gtggaccctt cggaaccatc
      301 aagcaccctg ccgaactggc tcacagcgct aacaacggtc ttgacatcgc tgttaggctt
      361 ttggagccac tcaaggcgga gttccctatt ttgagctacg ccgatttcta ccagttggct
      421 ggcgttgttg ccgttgaggt cacgggtgga cctaaggttc cattccaccc tggaagagag
      481 gacaagcctg agccaccacc agagggtcgc ttgcccgatg ccactaaggg ttctgaccat
      541 ttgagagatg tgtttggcaa agctatgggg cttactgacc aagatatcgt tgctctatct
      601 gggggtcaca ctattggagc tgcacacaag gagcgttctg gatttgaggg tccctggacc
      661 tctaatcctc ttattttcga caactcatac ttcacggagt tgttgagtgg tgagaaggaa
      721 ggtctccttc agctaccttc tgacaaggct cttttgtctg accctgtatt ccgccctctc
      781 gttgataaat atgcagcgga cgaagatgcc ttctttgctg attacgctga ggctcaccaa
      841 aagctttccg agcttgggtt tgctgatgcc
//
