LOCUS       sequence-59285-e        1007 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PAP39A
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgcgc
      121 accggctacc gagtcttctc ggccaactcc acggccgcct gcacggagct ggccaagcgc
      181 atcacagagc gccttggtgc tgaattgggg aagtctgttg tatatcaaga gaccaatgga
      241 gaaacaagag ttgaaataaa agaatctgtt cgtggccaag atattttcat tatacagaca
      301 atacccagag atgtgaatac agctgtgatg gagttgctca tcatggctta cgcactgaag
      361 actgcctgtg ccaggaacat tattggggtc atcccctact tcccctacag caagcagagc
      421 aagatgagga agaggggttc cattgtgtgc aagctgctag catccatgct ggcgaaagca
      481 ggtttaactc acattatcac tatggatctt catcaaaagg aaatacaagg ctttttcagc
      541 tttcctgtgg acaaccttag agcctcacct ttcctgcttc agtatatcca ggaagaaatt
      601 ccaaattaca gaaatgcagt cattgtagct aagtctcctg atgctgcaaa gagggcccag
      661 tcctatgcgg agagactgcg tctgggtttg gccgtcattc acggggaagc tcagtgcacg
      721 gaactggaca tggacgatgg tcgtcactcc ccgcctatgg tcaaaaatgc tactgtgcac
      781 ccaggcctgg agttgccatt gatgatggcc aaagagaagc caccgataac tgtagttgga
      841 gatgttggag gccgcatcgc aatcatcgtg gatgacatta ttgacgatgt ggagagtttt
      901 gttgctgccg cggagatcct gaaagagaga ggcgcctata agatctatgt tatggccacc
      961 cacggcatcc tgtctgcaga ggcccctcgc ctgattgagg agtcctc
//
