LOCUS       sequence-59298-e         879 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Yeast expression of VPH2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Hughes AL, Gottschling DE
  TITLE     An early age increase in vacuolar pH limits mitochondrial function 
            and lifespan in yeast.
  JOURNAL   Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 
            2012 Nov 21.
  PUBMED    23172144
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11654"; journalName: "Nature"; date: "2012-12-13- 
            13"; volume: "492"; issue: "7428"; pages: "261-5"
COMMENT     Sequence Label: pAG306-GPD-VPH2 chr I
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..74
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     58..74
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    82..106
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(761..785)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(818..834)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(819..835)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(853..871)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 cttttatagt tagtcttttt tttagtttta aaacaccaga acttagtttc gacggattct
       61 agaactagtg gatcccccat cacaagtttg tacaaaaaag caggctccac catgttcgaa
      121 attaaactga atgatagaat aactgaattt ctgcgaaagt tcaagaatag cgcgaaatca
      181 aacgaaggaa ttgatgagga cattgatttg ttcctgaagc gacacgcaat acctatgcag
      241 tcacttcttt tttatgtgaa agagtataga aaagacagtg atctccaatg ctctatcaaa
      301 gaacttttga aacctttgga gttcgaattc aagcccaagg ccgtcagagg tttacactat
      361 tcagaggatt tcaagaaaaa attagagttt ttgaaatatc aggaacaaga acttgaatat
      421 caaagtatgg tcaagagaag caagtctgtc ttttcacttc aagaagacga tgaactcacg
      481 ccttcacaaa ttaataaaca gattaaggaa caagttacca ctgttttcaa cgttcttgta
      541 agtgtcatat cggttgttgt ggccatttgg tactggaccg gatcttcaac aaactttccg
      601 gtccatgttc gtcttttgtt gtgcttgttc tttggaatat tggtcttagt agcagatgtg
      661 gttgtgtata acagctatct gaaaaaactc gaagaagcaa aagtaaaaga aaaaacaaag
      721 gttgagaaaa agaaagttct aagcaagatt acactgtagg acccagcttt cttgtacaaa
      781 gtggtgatgg gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgagtcat
      841 gtaattagtt atgtcacgct tacattcacg ccctccccc
//
