LOCUS       sequence-59304-e         973 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EPB41L3A
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgaag
      121 cctaaaagca tgcagtgcaa agtgatactt ctcgatggat cagaatatac ctgtgatgta
      181 gagaaacgct ccagaggaca agtgctgttt gataaagtgt gtgaacactt gaacttgcta
      241 gagaaagact actttgggct tacgtatcga gatgctgaaa accagaagaa ttggttggac
      301 cctgctaagg aaataaaaaa acaggttcga agtggtgctt ggcacttttc atttaatgtg
      361 aaattttatc caccagaccc tgcccaacta tctgaagata tcaccaggta ctacctctgc
      421 ttgcagttgc gagatgacat cgtgtccgga aggctgccct gctcctttgt taccctggcc
      481 ttgctgggct cctacactgt ccagtcagag ctcggagact atgacccaga tgaatgtggg
      541 agcgattaca ttagtgagtt ccgctttgca ccaaaccaca ctaaagaact ggaagacaaa
      601 gtgatcgagc tgcacaagag ccacagagga atgacgccag cagaagcaga gatgcatttc
      661 ttggaaaatg ccaaaaaatt atcaatgtat ggggtagatt tacatcatgc taaggactca
      721 gaaggggtag aaattatgtt aggagtttgt gcaagtggtc tgttgatata tcgcgaccgg
      781 ctgcgaataa acagatttgc ctggcccaag gttctaaaga tttcatacaa acggaacaac
      841 ttttacatta agatccggcc gggagagttt gaacaatttg aaagcaccat tgggtttaag
      901 ctgccaaacc atcgagctgc caagcgttta tggaaagtat gtgttgagca tcatacattt
      961 ttcagactac tgt
//
