LOCUS       sequence-59390-e         951 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CTHA
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catgcaggaa
      121 aaagacgcct cctcacaagg tttcctgcca cacttccaac atttcgccac gcaggcgatc
      181 catgtgggcc aggatccaga gcaatggacc tccagggctg tagtgccccc catctcactg
      241 tccaccacgt tcaagcaagg ggcgcctggc cagcactcgg gttttgaata tagccgttct
      301 ggaaatccca ctaggaattg ccttgaaaaa gcagtggcag cactggatgg ggctaagtac
      361 tgtttggcct ttgcttcagg tttagcagcc actgtaacta ttacccatct tttaaaagca
      421 ggagaccaaa ttatttgtat ggatgatgtg tatggaggta caaacaggta cttcaggcaa
      481 gtggcatctg aatttggatt aaagatttct tttgttgatt gttccaaaat caaattacta
      541 gaggcagcaa ttacaccaga aaccaagctt gtttggatcg aaacccccac aaaccccacc
      601 cagaaggtga ttgacattga aggctgtgca catattgtcc ataagcatgg agacattatt
      661 ttggtcgtgg ataacacttt tatgtcacca tatttccagc gccctttggc tctgggagct
      721 gatatttcta tgtattctgc aacaaaatac atgaatggcc acagtgatgt tgtaatgggc
      781 ctggtgtctg ttaattgtga aagccttcat aatagacttc gtttcttgca aaactctctt
      841 ggagcagttc catctcctat tgattgttac ctctgcaatc gaggtctgaa gactctacat
      901 gtccgaatgg aaaagcattt caaaaacgga atggcagttg cccagttcct g
//
