LOCUS       sequence-59400-e         966 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIP4K2AA
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgga
      121 cccgctgctc agcgtcctca tgtggggggt aaaccactcg atcaatgaac tgagccatgt
      181 tcaaatccct gttatgttga tgccagatga cttcaaagcc tattcaaaaa taaaggtgga
      241 caatcacctt tttaacaaag aaaacatgcc gagccatttc aagtttaagg aatactgccc
      301 gatggtcttc cgtaacctgc gggagaggtt tggaattgat gatcaagatt tccagaattc
      361 cctgaccagg agcgcacccc tccccaacga ctcccaggcc cgcagtggag ctcgttttca
      421 cacttcctac gacaaaagat acatcatcaa gactattacc agtgaagacg tggccgaaat
      481 gcacaacatc ctgaagaaat accaccagta catagtggaa tgtcatggga tcacccttct
      541 tccccagttc ttgggcatgt accggcttaa tgttgatgga gttgaaatat atgtgatagt
      601 tacaagaaat gtattcagcc accgtttgtc tgtgtatagg aaatacgact taaagggctc
      661 tacagtggct agagaagcta gtgacaaaga aaaggccaaa gaactgccaa ctctgaaaga
      721 taatgatttc attaatgagg gccaaaagat ttatattgat gacaacaaca agaaggtctt
      781 cctggaaaaa ctaaaaaagg atgttgagtt tctggcccag ctgaagctca tggactacag
      841 tctgctggtg ggaattcatg atgtggagag agccgaacag gaggaagtgg agtgtgagga
      901 gaacgatggg gaggaggagg gcgagagcga tggcacccac ccggtgggaa cccccccaga
      961 tagccc
//
