LOCUS       sequence-60172-e         954 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIK3C3A
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgtc
      121 tgaccacgat ctgaaaccca atgctgccac gagagatcag ttaaatatta ttgtgagtta
      181 tccaccaacc aagcaactta catatgaaga acaagatctt gtttggaagt ttagatatta
      241 tcttacgaat caagaaaaag ccttgacaaa attcttgaaa tgtgttaatt gggatctacc
      301 tcaagaggcc aaacaggcct tggaacttct gggaaaatgg aagccgatgg atgtagagga
      361 ctccttggag ctgttatcct ctcattacac caacccaact gtgaggcgtt atgctgttgc
      421 ccggttgcga caggccgatg atgaggattt gttgatgtac ctattacaat tggtccaggc
      481 tctcaaatat gaaaattttg atgatataaa gaatggattg gaacctacca agaaggatag
      541 tcagagttca gtgtcagaaa atgtgtcaaa ttctggaata aattctgcag aaatagatag
      601 ctcccaaatt ataaccagcc cccttccttc agtctcttca cctcctcctg catcaaaaac
      661 aaaagaagtt ccagatggcg aaaatctgga acaagatctc tgtaccttct tgatatcgag
      721 agcctgcaaa aactcaacac tggctaatta tttatactgg tatgtgatag tggaatgtga
      781 agatcaagat actcagcaga gagatccaaa gacccatgag atgtacttga acgtaatgag
      841 aagattcagc caagcattgt tgaagggtga taagtctgtc agagttatgc gttctttgct
      901 ggctgcacaa cagacatttg tagatcggtt ggtgcatcta atgaaggcag taca
//
