LOCUS       sequence-60607-e         880 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-ATAG
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(600..616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(600..616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(613..635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    624..640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(648..654,655..672,673..678))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    693..714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(831..848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gccctctaga tgcatgctcg agcggccgcc agtgtgatgg atatctgcag aattcgccct
       61 tagtatcttt cctgtgccca cgtctctgag cccgtgcgct tggcacagca ctggaaggag
      121 cctctgcacc gtttccaaag cctgttttcc gccgttattg ctcgcgattg ccacgacctg
      181 ctctggggtt aatccgtgcg cttggcacag cacgggaagc aggcgctgga cagtctcgag
      241 tgcctgtttc ccgccaatgt tggaggcgat tgccacgacc tgctctgggg tgaggccgtg
      301 cgcttggcac agcaccggca gcaatcgttg tacggtttcc aacgcctgtt tgccccctcc
      361 attcgaggca atggccacga cctgctctgg ggtgagtccg tgcgcttggc acagcacggg
      421 aagcaggcgc tggacagtct cgagtgcctg tttcccgcca atgttggagg cgattgccac
      481 gacctgctct ggggttaagc gagacgcgct aggacggcat ctatataagg gcgaattcca
      541 gcacactggc ggccgttact agtggatccg agctcggtac caagcttggc gtaatcatgg
      601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
//
