LOCUS       sequence-60696-e         851 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUDT3A
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(558..578)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(603..620)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             631..653
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    668..692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(692..711)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(693..711)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(795..814)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccgtatcc acctttactg tcatgagtag
      121 ccttgcctca atgtttcaaa atatgatgcc tgcacgggtt tgtgatactg cagcactttt
      181 atggcgtctt ctattttaaa ccattccctc ttccttccaa tgttaactga atcttcccag
      241 tcttccagca cttcagtgac aatgagcaca tagacatacg tcctgtgctt cctctcctgg
      301 ttctcaaaaa ttccaactaa tcttcccaat gtccctttta ctccagcctc ctcacagact
      361 tcacgaactg ctgccacact tggctcctcc tcgggctcca tgcctcctcc agggacaatc
      421 catctgtctg gatggcgact actgctcacg agtagcacct cctcctcgct ctcgctgcgg
      481 aaacacaggc atgcggcccg cttcttgtag ccgtcgccgt cgtaggtgcg ggtctggttc
      541 gacttgagct tcatcatgga ttggaagtac aggttctcgg tacccagatc tacaccagaa
      601 gaatgatgat gatgatggtg catatgtata tctccttctt aaagttaaac aaaattattt
      661 ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg
      721 gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      781 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      841 cgggctcatg a
//
