LOCUS       sequence-60750-e       11674 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11674)
  TITLE     Plasmids to assay for nuclease cleavage in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSoup 0800 (35S::REN)
FEATURES             Location/Qualifiers
     source          1..11674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(376..395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            618..727
                     /note="incP origin of transfer"
     CDS             760..1131
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      1258..1969
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    2469..2491
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        2553..2898
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2841..2861
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2917..3852
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    3907..4083
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4098..4463)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWKL"
     primer_bind     4232..4254
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4246..4263
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4247..4263
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4273..4292
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4273..4291
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4300..4407
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     4316..4332
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4317..4333
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4367..4383)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4367..4383)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4420..4440)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4420..4438)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4459..4475)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4483..4499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4507..4513,4514..4532,4533..4538))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4553..4574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4813..4837
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        complement(4904..4922)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4905..4922)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4940..4956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4964..4980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4988..4994,4995..5012,5013..5018))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5033..5054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5301..5306,5307..5323,5324..5329)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             5377..6567
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(5729..5748)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             7066..8214
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             9595..10566
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein of plasmid pSa"
                     /label=RepA
                     /translation="MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATM
                     PHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRS
                     QSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNP
                     KRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLR
                     AKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREV
                     LIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT"
     CDS             10961..11611
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 cccctttcca ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc
       61 ctagcgtcca agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcttccg
      121 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      181 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      241 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      301 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      421 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      481 cgcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
      541 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
      601 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
      661 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
      721 tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca
      781 accaggaagg gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt
      841 gaggaaaagg cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag
      901 ggctacaaaa tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat
      961 ggcgacctgg gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg
     1021 gcgcggttcg gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac
     1081 gagcttggca aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag
     1141 ccgctaaaac ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa
     1201 gaagagcgac ttcgcggagc tggtgaagta catcaccgac gagcaaggca agaccgagcg
     1261 cctttgcgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc
     1321 ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt
     1381 gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt
     1441 gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg
     1501 cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc
     1561 cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg
     1621 cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat
     1681 gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca
     1741 agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa
     1801 tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga
     1861 aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc
     1921 caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggcagtc
     1981 cttgccattg ccgggatcgg ggcagtaacg ggatgggcga tcagcccgag cgcgacgccc
     2041 ggaagcattg acgtgccgca ggtgctggca tcgacattca gcgaccaggt gccgggcagt
     2101 gagggcggcg gcctgggtgg cggcctgccc ttcacttcgg ccgtcggggc attcacggac
     2161 ttcatggcgg ggccggcaat ttttaccttg ggcattcttg gcatagtggt cgcgggtgcc
     2221 gtgctcgtgt tcgggggtgc gataaaccca gcgaaccatt tgaggtgata ggtaagatta
     2281 taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt
     2341 taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata
     2401 ttgacaatac tgataagata atatatcttt tatatagaag ataattcgag ctcggtaccc
     2461 ggggatcttg gcaggatata ttgtggtgta acgttatcgt acccctactc caaaaatgtc
     2521 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatttcg
     2581 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
     2641 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat
     2701 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     2761 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgacatctc cactgacgta
     2821 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
     2881 tttcatttgg agaggacagc ccaagctcta gccaccatga cttcgaaagt ttatgatcca
     2941 gaacaaagga aacggatgat aactggtccg cagtggtggg ccagatgtaa acaaatgaat
     3001 gttcttgatt catttattaa ttattatgat tcagaaaaac atgcagaaaa tgctgttatt
     3061 tttttacatg gtaacgcggc ctcttcttat ttatggcgac atgttgtgcc acatattgag
     3121 ccagtagcgc ggtgtattat accagacctt attggtatgg gcaaatcagg caaatctggt
     3181 aatggttctt ataggttact tgatcattac aaatatctta ctgcatggtt tgaacttctt
     3241 aatttaccaa agaagatcat ttttgtcggc catgattggg gtgcttgttt ggcatttcat
     3301 tatagctatg agcatcaaga taagatcaaa gcaatagttc acgctgaaag tgtagtagat
     3361 gtgattgaat catgggatga atggcctgat attgaagaag atattgcgtt gatcaaatct
     3421 gaagaaggag aaaaaatggt tttggagaat aacttcttcg tggaaaccat gttgccatca
     3481 aaaatcatga gaaagttaga accagaagaa tttgcagcat atcttgaacc attcaaagag
     3541 aaaggtgaag ttcgtcgtcc aacattatca tggcctcgtg aaatcccgtt agtaaaaggt
     3601 ggtaaacctg acgttgtaca aattgttagg aattataatg cttatctacg tgcaagtgat
     3661 gatttaccaa aaatgtttat tgaatcggac ccaggattct tttccaatgc tattgttgaa
     3721 ggtgccaaga agtttcctaa tactgaattt gtcaaagtaa aaggtcttca tttttcgcaa
     3781 gaagatgcac ctgatgaaat gggaaaatat atcaaatcgt tcgttgagcg agttctcaaa
     3841 aatgaacaat aattctaggg tacgctgaaa tcaccagtct ctctctacaa atctatctct
     3901 ctctattttc tccataaata atgtgtgagt agtttcccga taagggaaat tagggttctt
     3961 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
     4021 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
     4081 atcgataaca ttaacgctta caatttccat tcgccattca ggctgcgcaa ctgttgggaa
     4141 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     4201 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     4261 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag
     4321 gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cactagttct
     4381 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     4441 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4501 tccacacaac atacgagccg gaaghcataa agtgtaaagc ctggggtgcc taatgagtga
     4561 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4621 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4681 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4741 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4801 acatgaaggc cttgacagga tatattggcg ggtaaactaa gtcgctgtat gtgtttgttt
     4861 gagatcctct agagtcgacc tgcaggcatg caagcttgag tattctatag tgtcacctaa
     4921 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4981 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5041 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5101 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5281 acatgaatta attctcatgt ttgacagctt atcatcgatt agctttaatg cggtagttta
     5341 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
     5401 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
     5461 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
     5521 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
     5581 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
     5641 atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt ggccggcatc
     5701 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     5761 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     5821 gtggccgggg gactgttggg cgccatctcc ttacatgcac cattccttgc ggcggcggtg
     5881 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     5941 cgccgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     6001 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     6061 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     6121 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     6181 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     6241 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     6301 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     6361 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     6421 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     6481 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     6541 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca
     6601 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc
     6661 cttgtatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg
     6721 attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac ctagatgtgg
     6781 cgcaacgatg ccggcgacaa gcaggagcgc accgacttct tccgcatcaa gtgttttggc
     6841 tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt cgtgcagggc
     6901 aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac cgacttcatt
     6961 gccgataagg tggattatct ggacaccaag gcaccaggcg ggtcaaatca ggaataaggg
     7021 cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt
     7081 gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga ggatgccgaa
     7141 accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg
     7201 atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc tccccctgcc
     7261 ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt
     7321 ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc
     7381 gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt gctgaaacac
     7441 acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc gtggccggac
     7501 acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac gcgcaacaag
     7561 aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag
     7621 atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg
     7681 ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt ctacgagctt
     7741 tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga ggaatgcctg
     7801 tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca cctggaatcg
     7861 gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag
     7921 gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac gaaattcata
     7981 tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta tttcagctcg
     8041 caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg atcggattcc
     8101 acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc
     8161 ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg ctagggcctt
     8221 gtggggtcag ttccggctgg gggttcagca gccagcgctt tactggcatt tcaggaacaa
     8281 gcgggcactg ctcgacgcac ttgcttcgct cagtatcgct cgggacgcac ggcgcgctct
     8341 acgaactgcc gataaacaga ggattaaaat tgacaattgt gattaaggct cagattcgac
     8401 ggcttggagc ggccgacgtg caggatttcc gcgagatccg attgtcggcc ctgaagaaag
     8461 ctccagagat gttcgggtcc gtttacgagc acgaggagaa aaagcccatg gaggcgttcg
     8521 ctgaacggtt gcgagatgcc gtggcattcg gcgcctacat cgacggcgag atcattgggc
     8581 tgtcggtctt caaacaggag gacggcccca aggacgctca caaggcgcat ctgtccggcg
     8641 ttttcgtgga gcccgaacag cgaggccgag gggtcgccgg tatgctgctg cgggcgttgc
     8701 cggcgggttt attgctcgtg atgatcgtcc gacagattcc aacgggaatc tggtggatgc
     8761 gcatcttcat cctcggcgca cttaatattt cgctattctg gagcttgttg tttatttcgg
     8821 tctaccgcct gccgggcggg gtcgcggcga cggtaggcgc tgtgcagccg ctgatggtcg
     8881 tgttcatctc tgccgctctg ctaggtagcc cgatacgatt gatggcggtc ctgggggcta
     8941 tttgcggaac tgcgggcgtg gcgctgttgg tgttgacacc aaacgcagcg ctagatcctg
     9001 tcggcgtcgc agcgggcctg gcgggggcgg tttccatggc gttcggaacc gtgctgaccc
     9061 gcaagtggca acctcccgtg cctctgctca cctttaccgc ctggcaactg gcggccggag
     9121 gacttctgct cgttccagta gctttagtgt ttgatccgcc aatcccgatg cctacaggaa
     9181 ccaatgttct cggcctggcg tggctcggcc tgatcggagc gggtttaacc tacttccttt
     9241 ggttccgggg gatctcgcga ctcgaaccta cagttgtttc cttactgggc tttctcagcc
     9301 ccagatccta gaggatcgag cggcatttca ttcaggcacg taccaccgag gcgctacaag
     9361 tcgccagaga gcgcggcaag acgctcgggc gacccaaggg cagcaaatcg agcgccttga
     9421 agctggacag ccgtattgat gaagtacagg catacgtgaa ccttggcttg ccgcaaagtc
     9481 gcgcagccga gttgttaggc gtcagccctc acaccttgcg cctgttcatc aaacgccgga
     9541 acatcaaacc cacaaacact agaccaacca tcaccatgcc ggggagggaa caacatgcct
     9601 aagaacaaca aagcccccgg ccatcgtatc aacgagatca tcaagacgag cctcgcgctc
     9661 gaaatggagg atgcccgcga agctggctta gtcggctaca tggcccgttg ccttgtgcaa
     9721 gcgaccatgc cccacaccga ccccaagacc agctactttg agcgcaccaa tggcatcgtc
     9781 accttgtcga tcatgggcaa gccgagcatc ggcctgccct acggttctat gccgcgcacc
     9841 ttgcttgctt ggatatgcac cgaggccgtg cgaacgaaag accccgtgtt gaaccttggc
     9901 cggtcgcaat cggaatttct acaaaggctc ggaatgcaca ccgatggccg ttacacggcc
     9961 acccttcgca atcaggcgca acgcctgttt tcatccatga tttcgcttgc cggcgagcaa
    10021 ggcaatgact tcggcattga gaacgtcgtc attgccaagc gcgcttttct attctggaat
    10081 cccaagcggc cagaagatcg ggcgctatgg gatagcaccc tcaccctcac aggcgatttc
    10141 ttcgaggaag tcacccgctc accggttcct atccgaatcg actacctgca cgccttgcgg
    10201 cagtctccgc ttgcgatgga catttacacg tggctgacct atcgcgtgtt cctgttgcgg
    10261 gccaagggcc gccccttcgt gcaaatccct tgggtcgccc tgcaagcgca attcggctca
    10321 tcctatggca gccgcgcacg caactcgccc gaactggacg ataaggcccg agagcgggca
    10381 gagcgggcag cactcgccag cttcaaatac aacttcaaaa agcgcctacg cgaagtgttg
    10441 attgtctatc ccgaggcaag cgactgcatc gaagatgacg gcgaatgcct gcgcatcaaa
    10501 tccacacgcc tgcatgtcac ccgcgcaccc ggcaagggcg ctcgcatcgg cccccctccg
    10561 acttgaccag gccaacgcta cgcttggctt ggtcaaggat cgatctggcg ccggccagcg
    10621 agacgagcaa gattggccgc cgcccgaaac gatccgacag cgcgcccagc acaggtgcgc
    10681 aggcaaattg caccaacgca tacagcgcca gcagaatgcc atagtgggcg gtgacgtcgt
    10741 tcgagtgaac cagatcgcgc aggaggcccg gcagcaccgg cataatcagg ccgatgccga
    10801 cagcgtcgag cgcgacagtg ctcagaatta cgatcagggg tatgttgggt ttcacgtctg
    10861 gcctccggac cagcctccgc tggtccgatt gaacgcgcgg attctttatc actgataagt
    10921 tggtggacat attatgttta tcagtgataa agtgtcaagc atgacaaagt tgcagccgaa
    10981 tacagtgatc cgtgccgccc tggacctgtt gaacgaggtc ggcgtagacg gtctgacgac
    11041 acgcaaactg gcggaacggt tgggggttca gcagccggcg ctttactggc acttcaggaa
    11101 caagcgggcg ctgctcgacg cactggccga agccatgctg gcggagaatc atacgcattc
    11161 ggtgccgaga gccgacgacg actggcgctc atttctgatc gggaatgccc gcagcttcag
    11221 gcaggcgctg ctcgcctacc gcgatggcgc gcgcatccat gccggcacgc gaccgggcgc
    11281 accgcagatg gaaacggccg acgcgcagct tcgcttcctc tgcgaggcgg gtttttcggc
    11341 cggggacgcc gtcaatgcgc tgatgacaat cagctacttc actgttgggg ccgtgcttga
    11401 ggagcaggcc ggcgacagcg atgccggcga gcgcggcggc accgttgaac aggctccgct
    11461 ctcgccgctg ttgcgggccg cgatagacgc cttcgacgaa gccggtccgg acgcagcgtt
    11521 cgagcaggga ctcgcggtga ttgtcgatgg attggcgaaa aggaggctcg ttgtcaggaa
    11581 cgttgaagga ccgagaaagg gtgacgattg atcaggaccg ctgccggagc gcaacccact
    11641 cactacagca gagccatgta gacaacatcc cctc
//
