LOCUS       sequence-60955-e       10927 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-mIRBP156-CHER-RK-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..10927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     232..252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1873..2106
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2368..2485
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2544..2600
                     /label=MCS
                     /note="multiple cloning site"
     CDS             2915..2929
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     regulatory      2936..2945
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2942..3652
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3087..3105)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3355..3374
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      4025..4034
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4031..4033,4034..4036,4037..4750)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4031..4033,4034..4036,4037..4747)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4076..4097)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4337..4356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4684..4705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4763..5351
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4816..4836)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5234..5245)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6280..6504
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6637..6656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(7677..7694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7848..8436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7928..7947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8607..9398,9399..9467))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9230..9249
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9468..9572)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9640..9658
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9709..9731)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9817..9955
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9870..9889
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtggcccgag agctgcatcc ggagtatcta gatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgactga
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgc taatgggagt ttgttttggc
      181 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      241 gcggtaggcg tgtactctag aaggtctata taagcagagc tcgtttagtg aaccgtttat
      301 actacttatc tggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      481 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagtaaagcc agaggagatc
      541 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      601 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      661 gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa ggccaggggg
      721 aaagaaacaa tataaactaa aacatatagt atgggcaagc agggagctag aacgattcgc
      781 agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg gacagctaca
      841 accatccctt cagacaggat cagaagaact tagatcatta tataatacaa tagcagtcct
      901 ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag ataagataga
      961 ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca caggaaacaa
     1021 cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa tggtacatca
     1081 ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga aggctttcag
     1141 cccagaagta atacccatgt tttcagcatt atcagaagga gccaccccac aagatttaaa
     1201 taccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa aagagaccat
     1261 caatgaggaa gctgcagaat gggatagatt gcatccagtg catgcagggc ctattgcacc
     1321 aggccagatg agagaaccaa ggggaagtga catagcagga actactagca aattaagaga
     1381 acaatttgga aataataaaa caataatctt taagcaatcc tcaggagggg acccagaaat
     1441 tgtaacgcac agttttaatt gtggagggga atttttctac tgtaattcaa cacaactgtt
     1501 taatagtact tggtttaata gtacttggag tactgaaggg tcaaataaca ctgaaggaag
     1561 tgacacaatc acactcccat gcagaataaa acaatttata aacatgtggc aggaagtagg
     1621 aaaagcaatg tatgcccctc ccatcagtgg acaaattaga tgttcatcaa atattactgg
     1681 gctgctatta acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg
     1741 aggaggcgat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1801 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1861 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1921 cgcacggtca atgacgctga cggtacaggc cagacaatta ttgtctgata tagtgcagca
     1981 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     2041 gggcatcaaa cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     2101 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     2161 tgctagttgg agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg
     2221 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2281 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggctt gcggccggaa
     2341 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2401 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2461 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     2521 ggcggccgcg cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg
     2581 gtaccgcggg cccgggatcc ctacacagac atggctcagc aagatgagat gcaatgttcc
     2641 ttccgggtca aaccaccctg gcctcttctt cagagtccag ctcatgtgct tgaattagac
     2701 aggattaaag gcttagcaga gctggaagcc tcacatctaa ctcccacatt gagccccaga
     2761 ccttctgtct gcctgctaag aagggcaagg gagagaggca gctgcaggac cacagccttg
     2821 tacacacgga gctcttatcc aggagccaag gctctgggat ccaccggtcg ccgctagcgt
     2881 cgatcgaccc gggatcgata gtttaaacct gtacgacgat gacgataagg atcccgccac
     2941 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     3001 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     3061 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     3121 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     3181 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     3241 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     3301 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     3361 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     3421 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     3481 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     3541 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     3601 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aagaattcga
     3661 agcttgatcc ggctgctaac aaagcccgaa aggagtcgac ccgggatccg cggaatacta
     3721 gtgggcccca gaagcctggt ggttgtttgt ccttctcagg ggaaaagtga ggcggcccct
     3781 tggaggaagg ggccgggcag aatgatctaa tcggattcca agcagctcag gggattgtct
     3841 ttttctagca ccttcttgcc actcctaagc gtcctccgtg accccggctg ggatttagcc
     3901 tggtgctgtg tcagccccgg tctcccaggg gcttcccagt ggtccccagg aaccctcgac
     3961 agggcccggt ctctctcgtc cagcaagggc agggacgggc cacaggccaa gggcggtacc
     4021 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4081 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     4141 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     4201 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     4261 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     4321 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     4381 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     4441 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     4501 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     4561 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     4621 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4681 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     4741 gtacaagtaa aataacgcgt ataatcaacc tctggattac aaaatttgtg aaagattgac
     4801 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4861 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4921 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4981 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     5041 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     5101 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     5161 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     5221 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     5281 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     5341 cgcctccccg catcgatacc gtcgaccacg cgttatcgcg tactagtatc tagtgaattc
     5401 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg
     5461 ggcgaggggg atctcgactc tagaagcttg gtacctttaa gaccaatgac ttacaaggca
     5521 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5581 cagtttcgag ttggcgcgcc tgggagctca cggggacagc cccccccaaa gcccccaggg
     5641 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     5701 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     5761 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     5821 cctgggggat acggggaaaa agctttaggc tgaaagagag atttagaatg acaggcgcgc
     5881 ctgggagctc acggggacag ccccccccaa agcccccagg gatgtaatta cgtccctccc
     5941 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     6001 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     6061 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctggggga tacggggaaa
     6121 aagctttagg ctgaaagaga gatttagaat gacaggcgcg ccaactcgaa ttcattgcag
     6181 actttcactg aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6241 aactagggaa cccactgctt aagcctcaat aaagctcgac tgtgccttct agttgccagc
     6301 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6361 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6421 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6481 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     6541 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6601 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6661 ttctcgccac gttcgccggc tgtgcgggag aacggagttc tattatgact caaatcagtc
     6721 tccccaagca ttcggggatc agagttttta aggataactt agtgtgtagg gggccagtga
     6781 gttggagatg aaagcgtagg gagtcgaagg tgtccttttg cgccgagtca gttcctgggt
     6841 gggggccaca agatcggatg agccagttta tcaatccggg ggtgccagct gatccatgga
     6901 gtgcagggtc tgcaaaatat ctcaagcact gattgatctt aggttttaca atagtgatgt
     6961 taccccagga acaatttggg gaaggtcaga atcttgtagc ctgtagctgc atgactccta
     7021 aaccataatt tcttttttgt tttttttttt ttatttttga gacagggtct cactctgtca
     7081 cctaggctgg agtgcagtgg tgcaatcaca gctcactgca gcctcaacgt cgtaagctca
     7141 agcgatcctc ccacctcagc ctgcctggta gctgagacta caagcgacgc cccagttaat
     7201 ttttgtattt ttggtagagg cagcgttttg ccgtgtggcc ctggctggtc tcgaactcct
     7261 gggctcaagt gatccagcct cagcctccca aagtgctggg acaaccgggg ccagtcactg
     7321 cacctggccc taaaccataa tttctaatct tttggctaat ttgttagtcc tacaaaggca
     7381 gtctagtccc caggcaaaaa gggggtttgt ttcgggaaag ggctgttact gtctttgttt
     7441 caaactataa actaagttcc tcctaaactt agttcggcct acacccagga atgaacaagg
     7501 agagcttgga ggttagaagc acgatggaat tggttaggtc agatctcttt cactgtctga
     7561 gttataattt tgcaatggtg gttcaaagac tgcccgcttc tgacaccagt cgctgcatta
     7621 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggcgctcttc cgcttcctcg
     7681 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7741 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7801 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7861 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7921 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7981 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8041 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8101 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8161 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8221 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8281 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8341 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8401 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8461 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8521 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8581 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8641 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8701 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8761 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8821 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8881 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8941 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9001 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9061 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9121 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9181 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9241 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9301 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9361 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9421 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9481 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9541 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9601 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     9661 tttcgtcttc aagaactgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     9721 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     9781 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     9841 cgatagcgga gtgtactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     9901 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     9961 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    10021 acgccagcgc ggggaggcag agattgcagt aagctgagat cgcagcactg cactccagcc
    10081 tgggcgacag agtaagactc tgtctcaaaa ataaaataaa taaatcaatc agatattcca
    10141 atcttttcct ttatttattt atttattttc tattttggaa acacagtcct tccttattcc
    10201 agaattacac atatattcta tttttcttta tatgctccag ttttttttag accttcacct
    10261 gaaatgtgtg tatacaaaat ctaggccagt ccagcagagc ctaaaggtaa aaaataaaat
    10321 aataaaaaat aaataaaatc tagctcactc cttcacatca aaatggagat acagctgtta
    10381 gcattaaata ccaaataacc catcttgtcc tcaataattt taagcgcctc tctccaccac
    10441 atctaactcc tgtcaaaggc atgtgcccct tccgggcgct ctgctgtgct gccaaccaac
    10501 tggcatgtgg actctgcagg gtccctaact gccaagcccc acagtgtgcc ctgaggctgc
    10561 cccttccttc tagcggctgc ccccactcgg ctttgctttc cctagtttca gttacttgcg
    10621 ttcagccaag gtctgaaact aggtgcgcac agagcggtaa gactgcgaga gaaagagacc
    10681 agctttacag ggggtttatc acagtgcacc ctgacagtcg tcagcctcac agggggttta
    10741 tcacattgca ccctgacagt cgtcagcctc acagggggtt tatcacagtg cacccttaca
    10801 atcattccat ttgattcaca atttttttag tctctactgt gcctaacttg taagttaaat
    10861 ttgatcagag gtgtgttccc agaggggaaa acagtatata cagggttcag tactatcgca
    10921 tttcagg
//
