LOCUS       sequence-61161-e         830 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5. 4():21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact. 2005 Jul 5. 4():21."
COMMENT     Sequence Label: pQTEV-CGI12
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(45..59,60..65,66..82,83..89)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    97..113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             132..143
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             156..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             201..221
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa aaaatttatt
       61 tgctttgtga gcggataaca attataatag attcaattgt gagcggataa caatttcaca
      121 cagaattcat taaagaggag aaattaacta tgaaacatca ccatcaccat caccatagcg
      181 attacgacat ccccactact gagaatcttt attttcaggg atccaatgaa cagtcacaga
      241 agacacaaaa tatatccagc tttgattctg agctgtttct agaagaactg gatgaattgc
      301 ctccattgtc tccaatgcag ccaatttcag aggaagaggc tattcagatt attgcagacc
      361 ctccattgcc accagcttca ttcacacttc gagactatgt ggatcattct gagactctgc
      421 agaagttggt tcttctaggc gtggatttgt ccaagataga aaaacatcca gaagcagcaa
      481 acctccttct gagactggat tttgaaaaag acattaagca aatgcttctg tttcttaaag
      541 atgtgggtat agaggataac caactgggag cattcctgac aaaaaatcat gcaattttct
      601 ctgaagacct tgaaaatctg aagaccaggg tggcttatct gcattcaaaa aatttcagta
      661 aagcagatgt tgcacagatg gtcagaaaag caccattttt gctgaacttt tcagtggaaa
      721 gactggataa cagattggga ttttttcaga aagaacttga acttagtgtg aagaagacta
      781 gagatctggt agttcgtctc ccaaggctgc taactggaag tctggaaccc
//
