LOCUS       sequence-61227-e         825 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Shen RR, Zhou AY, Kim E, Lim E, Habelhah H, Hahn WC
  TITLE     IkappaB kinase epsilon phosphorylates TRAF2 to promote mammary 
            epithelial cell transformation.
  JOURNAL   Mol Cell Biol. 2012 Dec;32(23):4756-68. doi: 10.1128/MCB.00468-12. 
            Epub 2012 Sep 24.
  PUBMED    23007157
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00468-12"; journalName: "Mol Cell Biol"; date: 
            "2012-12"; volume: "32"; issue: "23"; pages: "4756-68"
COMMENT     Sequence Label: pWB-TRAF2 S102A
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14..37
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    44..68
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attcgccacc atggactaca aagacgatga cgacaagcaa ttgacaagtt tgtacaaaaa
       61 agttggcatg gctgcagcta gcgtgacccc ccctggctcc ctggagttgc tacagcccgg
      121 cttctccaag accctcctgg ggaccaagct ggaagccaag tacctgtgct ccgcctgcag
      181 aaacgtcctc cgcaggccct tccaggcgca gtgtggccac cggtactgct ccttctgcct
      241 ggccagcatc ctcagctctg ggcctcagaa ctgtgctgcc tgtgttcacg agggcatata
      301 tgaagaaggc atttctattt tagaaagcag ttcggccttc ccagataatg ctgcccgcag
      361 ggaggtggag gccctgccgg ccgtctgtcc cagtgatgga tgcacctgga aggggaccct
      421 gaaagaatac gagagctgcc acgaaggccg ctgcccgctc atgctgaccg aatgtcccgc
      481 gtgcaaaggc ctggtccgcc ttggtgaaaa ggagcgccac ctggagcacg agtgcccgga
      541 gagaagcctg agctgccggc attgccgggc accctgctgc ggagcagacg tgaaggcgca
      601 ccacgaggtc tgccccaagt tccccttaac ttgtgacggc tgcggcaaga agaagatccc
      661 ccgggagaag tttcaggacc acgtcaagac ttgtggcaag tgtcgagtcc cttgcagatt
      721 ccacgccatc ggctgcctcg agacggtaga gggtgagaaa cagcaggagc acgaggtgca
      781 gtggctgcgg gagcacctgg ccatgctact gagctcggtg ctgga
//
