LOCUS       sequence-61255-e         822 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Bird LE
  TITLE     High throughput construction and small scale expression screening of
            multi-tag vectors in Escherichia coli.
  JOURNAL   Methods. 2011 Sep;55(1):29-37. Epub 2011 Aug 10.
  PUBMED    21856427
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2011-09"; volume: "55(1)"; pages: "29-37. Epub 2011 Aug 10"
COMMENT     Sequence Label: pOPINTRX
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..112
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             133..150
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             151..474
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEI
                     ADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLD
                     ANLA"
     protein_bind    548..569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(584..589,590..607,608..614)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    622..638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     627..649
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     646..662
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     646..662
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(678..695)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(678..694)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(687..709)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
       61 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga
      121 tataccatgg cacaccatca ccaccatcac agcgataaaa ttattcacct gactgacgac
      181 agttttgaca cggatgtact caaagcggac ggggcgatcc tcgtcgattt ctgggcagag
      241 tggtgcggtc cgtgcaaaat gatcgccccg attctggatg aaatcgctga cgaatatcag
      301 ggcaaactga ccgttgcaaa actgaacatc gatcaaaacc ctggcactgc gccgaaatat
      361 ggcatccgtg gtatcccgac tctgctgctg ttcaaaaacg gtgaagtggc ggcaaccaaa
      421 gtgggtgcac tgtctaaagg tcagttgaaa gagttcctcg acgctaacct ggccagcagc
      481 ggtctggaag ttctgtttca gggtaccgtc gacccgactg gaaagcgggc agtgagcgca
      541 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      601 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      661 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      721 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      781 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tg
//
