LOCUS       sequence-61700-e         958 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pET-GST-TNRC6A-(935-984)-NES-mut
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(226..249)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(256..909)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(276..298)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             complement(922..939)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgctcatttg tcttgtaaca ttcctccaga aaggtccatc ttattgcttt gtacattttc
      121 ctctggtgag tctctcgaag cactggcgtt tgaagcctgt ttaacggctg gatttatcca
      181 cgcttcttct tgtttgtttc cacctttcat cattccaagc ttcaggggcc cctggaacag
      241 aacttccaga tccgattttg gaggatggtc gccaccacca aacgtggctt gccagccctg
      301 caaaggccat gctatatact tgctggattt caagtactta tcaatttgtg ggatagcttc
      361 aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc aggcacattg ggtccatgta
      421 taaaacaaca tcaagagcgt catacaacat gaagtcagga tgggttacat gatcaccatt
      481 taaatatgtt ttatgacata aacgatcttc gaacattttc agcatttcag gtagcttgct
      541 aagaaaatca actttgagag tttcaaagtc tttactatat gcaattctcg aaacaccgta
      601 tctaatatcc aaaaccgctc cttcaagcat tgaaatctct gcacgctctt ttggacaacc
      661 acccaacatg ttgtgcttgt cagctatata acgtatgatg gccatagact gtgttaattt
      721 aacatcacca tcaatataat aaggaagatt gggaaactcc aaacccaatt caaacttttt
      781 gtttcgccat ttatcacctt catcgcgctc atacaaatgc tcttcatatt tttcttcaag
      841 atattccaaa agaagtcgag tgggttgcac aaggccctta attttccaat aacctagtat
      901 aggggacatg ctagccatat ggctgccgcg cggcaccagg ccgctgctgt gatgatga
//
