LOCUS       sequence-61893-e         971 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Retroviral expression of DsRed in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ
  TITLE     Global protein stability profiling in mammalian cells.
  JOURNAL   Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489.
  PUBMED    18988847
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-11-7- 7"; volume: "322"; issue: 
            "5903"; pages: "918-23"
COMMENT     Sequence Label: MSCV-CMV-DsRed-IRES-d1EGFP
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(50..172,173..178,179..889,890..892,
                     893..895))
                     /codon_start=1
                     /product="EGFP destabilized by fusion to a mutated version 
                     of residues 422-461 of mouse ornithine decarboxylase, 
                     giving an in vivo half-life of ~1 hour"
                     /label=d1EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(221..242)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     570..589
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     829..850
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctct cgaggccgcc tacacattga
       61 tcctagcaga agcacaggct gcagggtgac ggtccatccc gctctcctgg gcacaagaca
      121 tgggcagcgt gccatcatcc tgcgccgcca ccgccggcgg gaagccatgg ctaagcttct
      181 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
      241 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
      301 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      361 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
      421 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
      481 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      541 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      601 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      661 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
      721 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
      781 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
      841 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggttg
      901 tggccatatt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct
      961 agacgttttt t
//
