LOCUS       sequence-62004-e         905 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             88..153
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      182..369
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(237..261)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        444..787
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(855..874)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tcgtacgctg caggtcgacg gatccccggg ttaattaacg agaatttata cttccaaggt
       61 tctggtggag gcggtggagg ctctagagac tacaaggacc acgacggtga ctacaaggac
      121 cacgacattg actacaagga tgacgatgac aagtgactcg aggcgcgcca cttctaaata
      181 agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
      241 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
      301 tttcctgtag gtcaggttgc tttctcaggt atagtatgag gtcgctctta ttgaccacac
      361 ctctaccggc agatccgcta gggataacag ggtaatatag atctgtttag cttgcctcgt
      421 ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg
      481 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
      541 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
      601 gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
      661 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
      721 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
      781 aacaaccatg ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga
      841 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
      901 ctatc
//
