LOCUS       sequence-62073-e         884 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pZC03 (pFA6a-TEV-6xGly-V5-His3MX)
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             88..129
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      158..345
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(213..237)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        420..763
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tcgtacgctg caggtcgacg gatccccggg ttaattaacg agaatttata cttccaaggt
       61 tctggtggag gcggtggagg ctctagaggt aagccaattc caaacccatt gttaggtttg
      121 gattctactt gactcgaggc gcgccacttc taaataagcg aatttcttat gatttatgat
      181 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
      241 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
      301 tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga
      361 taacagggta atatagatct gtttagcttg cctcgtcccc gccgggtcac ccggccagcg
      421 acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat
      481 gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac
      541 attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc
      601 agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa
      661 atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct
      721 tgctaggata cagttctcac atcacatccg aacataaaca accatggcag aaccagccca
      781 aaaaaagcaa aaacaaactg ttcaggagcg caaggcgttt atctcccgta tcactaatga
      841 aactaaaatt caaatcgcta tttcgctgaa tggtggttat attc
//
