LOCUS       sequence-62375-e         802 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses optogenetically controlled RhoA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Bugaj LJ, Choksi AT, Mesuda CK, Kane RS, Schaffer DV
  TITLE     Optogenetic protein clustering and signaling activation in mammalian
            cells.
  JOURNAL   Nat Methods. 2013 Feb 3. doi: 10.1038/nmeth.2360.
  PUBMED    23377377
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Feb 3. doi: 10.1038/nmeth.2360."
COMMENT     Sequence Label: pCry2PHR-mCh-RhoA
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      98..107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
       61 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tggtggcgac cggtggatcc
      121 cgggctgctg ctccgatcat gatctgtgct tcacgggttc ttgaaatagc tttagctagt
      181 agctcacgag ctgtgtcgat gtctacaatg ggtttcgcat agtttgttcc gagttccaca
      241 ccagaagctt tgagtacggt taaaggagcg tcccatggat gatggatcca ttcagttggc
      301 aatctcgcaa gctcgggaag ccattgcctt atgtactcac cttctgggtc atatttggcg
      361 ccttgtaacg cgggattgtc caagcgatca agctcgtggc catcggggat actcccagag
      421 atatactgcc agccaaggat gtcacattcc aaatcagcat ccaaaagtgt atcccagaaa
      481 tacttcattc cccatttcca tggaaggaga agaaacttca cagcaaagct tgaaacaatc
      541 actcttattc tgttatgcat ccatccggta gcccaaagct ctctcattcc ggcatccacc
      601 aacggataac cggtcctgcc ttgtctccag gccttgaact tatcaacatc agcatcccaa
      661 gggaaaaacc gaagatgact caacaacgat tgctcgtgag taaacgggaa gttgaaacat
      721 atataccgag aatactctct taaaccgatt cccctaagaa aaagatctgc actttcttct
      781 ccttcactgt tcttatctct tg
//
