LOCUS       sequence-62570-e         805 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Nevozhay D, Zal T, Balazsi G
  TITLE     Transferring a synthetic gene circuit from yeast to mammalian cells.
  JOURNAL   Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
  PUBMED    23385595
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-02-5"; volume: "4:1451. doi"; pages: " 
            10.1038/ncomms2471"
COMMENT     Sequence Label: pDN-D2irTN6kwh
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        93..140
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             159..557
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    715..796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(752..771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
       61 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
      121 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag
      181 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
      241 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
      301 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
      361 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
      421 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
      481 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
      541 atgtgtggga gggctaagca cttcgtggcc gaggagcagg actgacacgt gctacgagat
      601 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
      661 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
      721 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      781 gcattttttt cactgcattc tagtt
//
