LOCUS       sequence-63188-e         920 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, 
            Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, 
            Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J
  TITLE     A lentiviral-based functional proteomics approach identifies 
            chromatin remodelling complexes important for the induction of 
            pluripotency.
  JOURNAL   Mol Cell Proteomics. 2010 May;9(5):811-23.
  PUBMED    20305087
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2010-05"; volume: "9(5)"; pages: "811-23"
COMMENT     Sequence Label: pLD-puro-CnVA
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..131
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             141..161
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             174..194
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             207..224
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             237..260
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             297..320
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    402..526
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(551..556,557..574,575..581)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(701..720)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcagagctc gtttagtgac cgtcagatcg cctggagacg ccatccacgc tgtgctagca
       61 ccatggacta caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg
      121 acgatgacaa gcctgcagga gaaaatctgt acttccaggg gagaggaggc agcgagaacc
      181 tgtactttca ggggcctgca ggaagacatc accatcacca tcacagcggc ggaagatgga
      241 gccaccctca gttcgagaag ggaggaggaa gcggcggagg cagcggagga ggaagctgga
      301 gccacccgca gttcgagaaa gcgatcgcca gagagctgtt caacctgctg ggcgagaacc
      361 agccccccgt ggtgatcaag gcgatcgcca ccggtaagct tacaagtttg tacaaaaaag
      421 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
      481 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg
      541 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
      601 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
      661 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
      721 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
      781 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
      841 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
      901 cccttgttac accgttttcc
//
