LOCUS       sequence-63307-e         932 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-XOPS-tdTOM-MOPS-GFP
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(81..104)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(279..298)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     548..566
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(712..726)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ggtgctgccg gtgccatgcc ccaggaacag gtggtggcgg ccctcggagc gctcgtactg
       61 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta
      121 gtagccgggc agttgcacgg gcttcttggc catgtagatg gtcttgaact ccaccaggta
      181 gtggccgccg tccttcagct tcagggcctg gtggatctcg cccttcagca cgccgtcgcg
      241 ggggtacagg cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc
      301 gtcggggggg aagttggtgc cgcgcatctt caccttgtag atcagcgtgc cgtcctgcag
      361 ggaggagtcc tgggtcacgg tcaccagacc gccgtcctcg aagttcatca cgcgctccca
      421 cttgaagccc tcggggaagg acagcttctt gtaatcgggg atgtcggcgg ggtgcttcac
      481 gtacgccttg gagccgtaca tgaactgggg ggacaggatg tcccaggcga agggcagggg
      541 gccgcccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
      601 ctcgccctcg atctcgaact cgtggccgtt catggagccc tccatgcgca ccttgaagcg
      661 catgaactct ttgatgacct cctcgccctt gctcaccatg gtggcgggat ccttatcgtc
      721 atcgtcgtac aggtttaaac tatcgatccc gggtcgaacg ctagcctcga gatccctaga
      781 agcctgtgat cccaactgaa gctgttctac agtatgaccc actgtcccct cgtcctttta
      841 aagtcccacc tggagtaagc ttgcaacccc cagcgtcagc aacaaaggat ttagcgcact
      901 attaatcact gtaatccaag ctaaaattgc aa
//
