LOCUS       sequence-63568-e         954 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Retroviral expression of USP44 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald
            ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, 
            Elledge SJ
  TITLE     Anaphase initiation is regulated by antagonistic ubiquitination and 
            deubiquitination activities.
  JOURNAL   Nature. 2007 Apr 19;446(7138):876-81.
  PUBMED    17443180
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-04-19"; volume: "446(7138)"; pages: "876-81"
COMMENT     Sequence Label: pFS217-USP44_MSCV-TAP
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(162..186)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(202..228)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(241..264)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(286..702)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(302..318)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(334..356)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tcttccacag gcaacatggg agcagctaag gcaagcccaa atggactcgg tcgtgttgca
       61 gtccacacag tgccatttct gagggttgag gctggaatgg tcttgagcaa gctgcagctg
      121 cccaacatgt ttgcacgtat ccattgctag catggtgggc gcgccaactt ttttgtacaa
      181 acttgtttga taacctcgag cagcgtaatc tggaacatcg tatgggtatc ctccatcgag
      241 cttgtcatcg tcatccttgt agtccatggt ggcagatcta attccggcgc ctagagaagg
      301 agtgagggct ggataaaggg aggatcgagg cggggtcgaa cgaggaggtt caagggggag
      361 agacggggcg gatggaggaa gaggaggcgg aggcttaggg tgtacaaagg gcttgaccca
      421 gggagggggg tcaaaagcca aggcttccca ggtcacgatg taggggacct ggtctgggtg
      481 tccatgcggg ccaggtgaaa agaccttgat cttaacctgg gtgatgaggt ctcggttaaa
      541 ggtgccgtct cgcggccatc cgacgttaaa ggttggccat tctgcagagc agaaggtaac
      601 ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg atccgctcga catctttcca
      661 gtgacctaag gtcaaactta agggagtggt aacagtctgg ccctaatttt cagacaaata
      721 cagaaacaca gtcagacaga gacaacacag aacgatgctg cagcgctgca gcagacaaga
      781 cgcgcggctt cggttccaaa ccgaaagcaa aaattcagac ggaggcggga actgttttag
      841 gttctcgtct cctaccagaa ccacatatcc tgacggggtc ggattccaca tcgactccct
      901 tcctcaggtc gggccacaaa aacggccccc aaagtccctg ggacgtctcc cagg
//
