LOCUS       sequence-63705-e        1064 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY051
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(343..688)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(380..400)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtttgata gcttggcgcg
      301 cctcgagggg atcctctaga gtcgacctgc agatctcgtg cgtgtcctct ccaaatgaaa
      361 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
      421 tacgtcagtg gagatgtcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
      481 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagagag
      541 atcttgaatg atagcctttc ctttatcgca atgatggcat ttgtaggagc caccttcctt
      601 ttctactgtc ctttcgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
      661 cgaaattatc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
      721 gacatttttg gagtagacca gagtgtcgtg ctccaccatg ttgacgaaga ttttcttctt
      781 gtcattgagt cgtaaaagac tctgtatgaa ctgttcgcca gtcttcacgg cgagttctgt
      841 tagatcctcg atttgaatct tagactccat gcatggcctt agattcagta ggaactacct
      901 ttttagagac tccaatctct attacttgcc ttggtttatg aagcaagcct tgaatcgtcc
      961 atactggaat agtacttctg atcttgagaa atatgtcttt ctctgtgttc ttgatgcaat
     1021 tagtcctgaa tcttttgact gcatctttaa ccttcttggg aagg
//
