LOCUS       sequence-63991-e         961 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Westermann B, Neupert W
  TITLE     Mitochondria-targeted green fluorescent proteins: convenient tools 
            for the study of organelle biogenesis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2000 Nov;16(15):1421-7.
  PUBMED    11054823
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2000-11"; volume: "16(15)"; pages: "1421-7"
COMMENT     Sequence Label: pYX223-mtGFP
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(200..387)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(534..553)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     653..675
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(713..731)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     781..797
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     781..797
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gtatcatatg gtccagaaac cctatacctg tgtggacgtt aatcacttgc gattgtgtgg
       61 cctgttctgc tactgcttct gcctcttttt ctgggaagat cgagtgctct atcgctaggg
      121 gaccaccctt taaagagatc gcaatctgaa tcttggtttc atttgtaata cgctttacta
      181 gggctttctg ctctgtcatc tttgccttcg tttatcttgc ctgctcattt tttagtatat
      241 tcttcgaaga aatcacatta ctttatataa tgtataattc attatgtgat aatgccaatc
      301 gctaagaaaa aaaaagagtc atccgctagg tggaaaaaaa aaaatgaaaa tcattaccga
      361 ggcataaaaa aatatagagt gtactagagg aggccaagag taatagaaaa agaaaattgc
      421 gggaaaggac tgtgttatga cttccctgac taatgccgtg ttcaaacgat acctggcagt
      481 gactcctagc gctcaccaag ctcttaaaac gggaatttat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      601 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      661 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      721 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtgcggccgc
      781 tctagaacta gtggatcaat tccacggact atagactata ctagtatact ccgtctactg
      841 tacgatacac ttccgctcag gtccttgtcc tttaacgagg ccttaccact cttttgttac
      901 tctattgatc cagctcagca aaggcagtgt gatctaagat tctatcttcg cgatgtagta
      961 a
//
