LOCUS       sequence-64068-e         968 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ
  TITLE     The tumor suppressor CYLD regulates entry into mitosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74.
  PUBMED    17495026
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 May 22. 104(21):8869-74."
COMMENT     Sequence Label: HA-CYLD-C601A
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(131..151)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(131..149)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(170..186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(170..186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(183..205)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    194..210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(218..224,225..242,243..248))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    263..284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(401..418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(652..671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta attcgcggcc gcggcgccaa tgcattgggc ccggtaccca
      121 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
      181 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc gggagcataa
      241 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      301 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      361 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      961 gcggtgct
//
