LOCUS       sequence-64353-e         980 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pBabe_puro_DEST_Flag_PHC3
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..60
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             147..167
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(497..521)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(533..553)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        538..867
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      718..853
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(877..896)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccggatccc agtgtggtgg tacgtaggaa ttcatggact acaaagacga tgacgacaag
       61 caattgacaa gtttgtacaa aaaagttggc atggatactg aaccaaaccc gggaacatct
      121 tctgtgtcaa caacaaccag cagtaccacc accaccacca tcaccacttc ctcctctcga
      181 atgcagcagc cacagatctc tgtctacagt ggttcagacc gacatgctgt acaggcattg
      241 catcggcccc ccagctcagc tgctcagtac cttcagcaaa tgtatgcagc ccaacaacag
      301 cacttgatgc tgcatactgc agctcttcag cagcagcatt taagcagctc ccagcttcag
      361 agccttgctg ctgttcaggc aagtttgtcc agtggaagac catctacatc tcccacagga
      421 agtgtcacac agcagtcaag tatgtcccaa acgtctgtaa gttccctaaa tttttttttc
      481 ctggatttaa aattttaccc aactttcttg tacaaagtgg tctcgaccct gtggaatgtg
      541 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      601 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
      661 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
      721 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
      781 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
      841 ttttttggag gcctaggctt ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg
      901 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
      961 gactaccccg ccacgcgcca
//
