LOCUS       sequence-64383-e        9790 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Yang N, Zuchero JB, Ahlenius H, Marro S, Ng YH, Vierbuchen T, 
            Hawkins JS, Geissler R, Barres BA, Wernig M
  TITLE     Generation of oligodendroglial cells by direct lineage conversion.
  JOURNAL   Nat Biotechnol. 2013 May;31(5):434-9. doi: 10.1038/nbt.2564. Epub 
            2013 Apr 14.
  PUBMED    23584610
REFERENCE   2  (bases 1 to 9790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2564"; 
            journalName: "Nat Biotechnol"; date: "2013-05"; volume: "31"; issue:
            "5"; pages: "434-9"
COMMENT     Sequence Label: Tet-O-FUW-Sox10
FEATURES             Location/Qualifiers
     source          1..9790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1437..2025
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1490..1510)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1908..1919)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2028..2044)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2029..2045)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2550..2730
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2756..2773)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2762..2986
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3032..3460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3119..3138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3329..3350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3469..3489)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3474..3803
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3654..3789
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3716..3735
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3851..3898
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3917..4291
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4421..4542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4458..4477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4512..4531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4591..4607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4591..4607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4604..4626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4615..4631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4639..4645,4646..4663,4664..4669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4684..4705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4822..4839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4993..5581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5073..5092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5752..6543,6544..6612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6375..6394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6613..6717)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6792..6811)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6983..7362
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7364..7562
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7517..7537
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7580..7760
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7807..7932
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8425..8658
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8843..8887
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9185..9302
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9356..9374,9375..9397,9398..9416,9417..9439,9440..9458,
                     9459..9481,9482..9500,9501..9523,9524..9542,9543..9565,
                     9566..9584,9585..9607,9608..9626)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9659..9696
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9693..9717
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattcgccac catggccgag gaacaagacc tatcagaggt ggagctgagc cctgtgggct
       61 cggaggaacc ccgctgcctg tccccaggca gcgcgccgtc gctgggaccc gacggcggcg
      121 gcggtggctc gggcttgcga gccagcccgg ggcccggtga actgggcaag gtcaagaagg
      181 aacagcagga cggcgaggcg gacgatgaca agttccccgt gtgcatccgc gaggcggtca
      241 gccaggtgct cagcggctac gactggacgc tggtgcccat gcccgtgcgc gtcaacggtg
      301 ccagcaagag caagccgcac gtcaagaggc ccatgaacgc cttcatggtg tgggcacagg
      361 cggcacgcag aaagctagcc gaccagtacc ctcacctcca caatgctgag ctcagcaaga
      421 cactaggcaa gctctggagg ttgctgaacg aaagtgacaa gcgccccttc attgaggagg
      481 ctgagaggct ccggatgcag cacaaaaagg accatccgga ctacaagtac caacctcggc
      541 ggcggaagaa cgggaaggca gcccaggggg aggcagaatg cccaggcggg gaagccgagc
      601 aaggaggggc tgctgctatt caggctcact acaagagtgc ccacctggac caccggcacc
      661 cagaagaagg ctcccccatg tcagatggga acccagagca cccctcaggc cagagccatg
      721 gccccccaac ccctccaacc accccaaaga cagagctgca gtccggcaag gcagacccca
      781 aaagggatgg gcgctccttg ggggagggcg ggaagcccca catcgacttc ggcaacgtgg
      841 acatcgggga gatcagccac gaggtaatgt ccaacatgga aacctttgat gtgactgagc
      901 tggaccaata cctgccaccc aacgggcacc caggccatgt gggtagctac tcggcagctg
      961 gctacgggct gggcagtgcc ctggctgtgg ccagtggaca ctctgcctgg atctccaagc
     1021 caccaggtgt ggctctgccc acggtctcgc cccctggtgt ggatgccaaa gcccaggtga
     1081 agacagagac cacaggcccc cagggacccc cacactacac cgaccagccg tccacttccc
     1141 agatcgccta cacttccctc agtctgcccc actacggctc cgccttcccc tccatctcac
     1201 gaccccagtt tgactattct gaccatcagc cctcaggacc ctattatggc catgcaggcc
     1261 aggcctctgg cctctattca gccttttctt acatggggcc ctcccagcgg cccctctaca
     1321 ctgccatctc tgaccccagc ccctcagggc cccaatccca cagtcccaca cactgggagc
     1381 agccagtata tacgactcta tcccgacctt aggaattcga tatcaagctt atcgataatc
     1441 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     1501 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     1561 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     1621 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     1681 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     1741 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     1801 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     1861 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     1921 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     1981 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     2041 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     2101 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     2161 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     2221 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     2281 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     2341 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     2401 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     2461 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     2521 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     2581 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2641 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2701 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     2761 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2821 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2881 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2941 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3001 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     3061 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3121 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3181 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3241 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3301 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3361 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3421 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     3481 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3541 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3601 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3661 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3721 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     3781 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     3841 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     3901 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     3961 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     4021 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     4081 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     4141 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     4201 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     4261 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     4321 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4381 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     4441 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4501 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     4561 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4621 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4681 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4741 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4801 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5401 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5701 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     5761 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     5821 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     5881 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5941 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6001 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6061 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6121 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6181 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6241 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6301 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6361 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6421 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6481 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6541 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6601 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     6661 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     6721 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     6781 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     6841 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     6901 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     6961 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     7021 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     7081 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     7141 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     7201 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     7261 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     7321 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     7381 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     7441 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     7501 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     7561 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7621 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7681 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7741 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     7801 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     7861 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     7921 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     7981 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     8041 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     8101 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     8161 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     8221 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     8281 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8341 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8401 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     8461 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8521 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8581 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8641 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8701 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8761 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8821 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8881 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     8941 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     9001 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     9061 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     9121 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     9181 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9241 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9301 ttcgggttta ttacagggac agcagagatc cagtttggtt actcgagttt accactccct
     9361 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     9421 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     9481 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     9541 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     9601 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagt
     9661 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     9721 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     9781 cgcggccccg
//
