LOCUS       sequence-64479-e         842 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Rouhana L, Weiss JA, King RS, Newmark PA
  TITLE     PIWI homologs mediate histone H4 mRNA localization to planarian 
            chromatoid bodies.
  JOURNAL   Development. 2014 Jul;141(13):2592-601. doi: 10.1242/dev.101618. 
            Epub 2014 Jun 5.
  PUBMED    24903754
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.101618"; journalName: "Development"; date: "2014-07"; 
            volume: "141"; issue: "13"; pages: "2592-601"
COMMENT     Sequence Label: pBS-Histone H3 imperfect loop
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..55
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     40..56
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(627..647)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(627..645)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(666..682)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(666..682)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(679..701)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    690..706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(714..720,721..738,739..744))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    759..780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
       61 gcttgatatc gaattccgtt gctgtcgaac aaagcaaact gctcgtaaaa gtactggagg
      121 caaagctcca agaaaacaat tggctaccaa agctgctagg aagagcgcac cagcaactgg
      181 tggtgttaag aaacctcata gatatagacc aggaactgtt gctttaagag aaattcgacg
      241 ttatcaaaag agtaccgaac ttttaattag aaaactacca tttcaacgac tagttcgaga
      301 aattgcacaa gattttaaaa ctgatttacg atttcaaagt tcagctgttt cagctctaca
      361 agaggcaagt gaagcttatc ttgttggctt gtttgaagac accaatttat gtgcaattca
      421 tgccaaacga gtgacaatca tgccaaaaga tattcagtta gcaagaagaa taagaggaga
      481 aagagcttaa cagttgttat tatgtaaaag taaaaggctc tctttagggc cacaagtaat
      541 aattgtcaat agatttaaaa ttattctaaa aaaaaaaaaa aaaaatgcga gcggccgcca
      601 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      661 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      721 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      781 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      841 tg
//
