LOCUS       sequence-64775-e         881 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Balskus EP, Walsh CT
  TITLE     The genetic and molecular basis for sunscreen biosynthesis in 
            cyanobacteria.
  JOURNAL   Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637.
            Epub 2010 Sep 2.
  PUBMED    20813918
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1193637"; journalName: "Science"; date: 
            "2010-09-24- 24"; volume: "329"; issue: "5999"; pages: "1653-6"
COMMENT     Sequence Label: pET28b-NpR5600
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgagtaat gttcaagcat cgtttgaagc aacggaagct
      121 gaattccgcg tggaaggtta cgaaaaaatt gagtttagtc ttgtctatgt aaatggtgca
      181 tttgatatca gtaacagaga aattgcagac agctatgaga agtttggccg ttgtctgact
      241 gtgattgatg ctaatgtcaa cagattatat ggcaagcaaa tcaagtcata ttttagacac
      301 tatggtattg atctgaccgt agttcccatt gtgattactg agcctactaa aacccttgca
      361 acctttgaga aaattgttga tgctttttct gactttggtt taatccgcaa ggaaccagta
      421 ttagtagtgg gtggtggttt aaccactgat gtagctgggt ttgcgtgtgc tgcttaccgt
      481 cgtaagagta actatattcg ggttccgaca acgctgattg gtctgattga tgcaggtgta
      541 gcgattaagg ttgcagtcaa ccatcgcaag ttaaaaaatc gcttgggtgc atatcatgct
      601 cctttgaaag tcatcctcga tttctccttc ttgcaaacat taccaacggc tcaagttcgt
      661 aatgggatgg cagagttggt caaaattgct gttgtggcga actctgaagt ctttgaattg
      721 ttgtatgaat atggcgaaga gttgctttcc actcactttg gatatgtgaa tggtacaaag
      781 gaactgaaag cgatcgcaca taaactcaat tacgaggcaa taaaaactat gctggagttg
      841 gaaactccaa acttgcatga gttagacctc gatcgcgtca t
//
