LOCUS       sequence-64907-e         813 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Crefcoeur RP, Yin R, Ulm R, Halazonetis TD
  TITLE     Ultraviolet-B-mediated induction of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800.
  PUBMED    23653191
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1779. doi"; pages: " 
            10.1038/ncomms2800"
COMMENT     Sequence Label: pIRESN2 H2B-GFP-uvr8at(2x)
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             161..538
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      551..560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(596..624)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 ttggtaccga gctcggatcg atatctgcgg cctagctagc gctaccggac tcagatctcg
      121 agctcaagct tcgaattctg cagtcgacgg taccgccacc atgccagagc cagcgaagtc
      181 tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg
      241 caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa
      301 gcaggtccac cctgacaccg gcatttcgtc caaggctatg ggcatcatga attcgtttgt
      361 gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg
      421 ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt
      481 ggccaagcac gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaagga
      541 cccaccggtc gccaccatgg aatcctctag caaaggagaa gaactcttca ctggagttgt
      601 cccaattctt gttgaattag atggtgatgt taacggccac aagttctctg tcagtggaga
      661 gggtgaaggt gatgcaacat acggaaaact taccctgaag ttcatctgca ctactggcaa
      721 actgcctgtt ccttggccaa cactagtcac tactctgtgc tatggtgttc aatgcttttc
      781 aagatacccg gatcatatga aacggcatga ctt
//
