LOCUS       sequence-65010-e         670 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Arch voltage indicator with double mutations D95N-D106E (Arch-EEN), 
            with faster kinetics and greater fluorescence dynamic range than 
            Arch-D95N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Gong Y, Li JZ, Schnitzer MJ
  TITLE     Enhanced Archaerhodopsin Fluorescent Protein Voltage Indicators.
  JOURNAL   PLoS One. 2013 Jun 19;8(6):e66959.
  PUBMED    23840563
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-06-19"; volume: "8(6)"; pages: "e66959"
COMMENT     Sequence Label: pLenti-Arch-EEN
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..322)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(113..134)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     462..481
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgtaatccag aggttgatta tcgataagct tgatatcgaa ttctcattac acctcgttct
       61 cgtagcagaa cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      121 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc
      181 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      241 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
      301 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      361 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      421 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      481 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      541 cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag ccgaaggtgg
      601 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
      661 gcttgccgta
//
