LOCUS       sequence-65033-e       14067 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14067)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 14067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pCasper4-tubulin-IVS-Syn21-QSco-p10
FEATURES             Location/Qualifiers
     source          1..14067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..586)
                     /label=P element 5' end
     CDS             complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
                     3507..3780,4175..4246))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4706..4724
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     intron          7506..7572
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    join(11075..11276,11277..11307)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(11844..11863)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11963..11985
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12023..12041)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12109..12213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12214..12282,12283..13074)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12432..12451)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13245..13833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13734..13753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     1381 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     1441 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     1501 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     1561 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     1621 atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg
     1681 gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata
     1741 ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga
     1801 agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca
     1861 gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt gcccagaaag tatgtgtcac
     1921 agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct
     1981 aaatcgaatc gattcattag aaagttagta aattattaat atgcaaatgt attctaaaca
     2041 agacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag
     2101 atggctccgt tgatattcat cacgcccact tgcgtgagtt gttggcccaa aaagatgagg
     2161 ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcat
     2221 atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt ttctgcaaaa ctataaacta
     2281 tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctaa ctaaaatcta
     2341 gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac
     2401 cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga
     2461 tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc
     2521 cggctgctcc agtggcttct ccaaattttt ggtggccaac aactgctcca tatcccgggc
     2581 tactttgcta atagcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc
     2641 ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg
     2701 acactgggca cccacgctgg ataggagttg agatgtaatg taatgctaga tacccttaat
     2761 aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag
     2821 ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg
     2881 gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga
     2941 cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg ctcatcgcag atcagaagcg
     3001 gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac
     3061 ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct
     3121 ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca
     3181 ccatggcctg gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct
     3241 gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca
     3301 gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca
     3361 gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg
     3421 ccacgccgca aactgagttt caaattggta attggaccct ttattaagat ttcacacaga
     3481 tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat
     3541 gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc
     3601 cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc
     3661 tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggcagga gcgtgccgta
     3721 gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc
     3781 ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac
     3841 aaaacgaagt atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat
     3901 cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatgtagtcg aaaatgaatg
     3961 cctttagatg tcttgatcat gatatgatct taaaaattgt cttatatagc gagcacagct
     4021 accagaataa tctgtttcgt gtcactattt gtttgtgcga ttgcggtttg ggatttttgt
     4081 gggtcgcagt tctcacgccg cagacaattt gatgttgcaa tcgcagttcc tatagatcaa
     4141 gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt
     4201 gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc
     4261 acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa
     4321 caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa
     4381 ccgcttggag cttcgtcacg aaaccgctga caaagtgcaa ctgaaggcgg acattgacgc
     4441 taggtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg
     4501 atatttctgt ggacagagaa ggaggcaaac agcgctgact ttgagtggaa tgtcattttg
     4561 agtgagaggt aatcgaaaga acctggtact tcaaataccc ttggatcgaa gtaaatttaa
     4621 aactgatcag ataagttcaa tgatgtccag tgcagtaaaa aataaaaaaa aaatatgttt
     4681 ttttaaatct acattctcca aaaaagggtt ttattaactt acatacatac tagaattcga
     4741 gctcaatgca gtactaaccc gacttaccga tgtcgacgaa gaggtccagg tccgaggatc
     4801 gatttccaat gcctgtgatg cgggacccga acttgtagac tcgcagtggt tgtttgggaa
     4861 agcccggcga caaaagcttg cacaggtcct gttcgataac gtcgtactcg ggaatcacct
     4921 ttaacttgtc cgctattggg tatttgtcct taaacacatt cgtaatctct cccacaatca
     4981 ttgccctggc ctctgcaatg taaaaagtat ttataaaata tcgctggctc taaagaaaac
     5041 atattaatca aagtattgag gctttatcaa tctaacattt acaagtagtc agaccacaat
     5101 ttttagactg cacttctgcg taagtaaatg aaatgaaatg gtgacctact cttgggtagc
     5161 cgtactgtta tatgctgacg agccttcacc tgggcctgct ttttctcctg tttcgtctga
     5221 tgcgtcggat gcggctggac tggctgattg ttgggattgg gttttggatg ctgctccgaa
     5281 aggtgggcca cagcttttgc gatggtggtg cccactatgc ggttgctgca ggaactacag
     5341 ctgaatgttc gctttattgt ctgcagctcc tggggctgga cgaaggccac gccctcaccc
     5401 ggtgcggcca tctcctgcgg caaaacgtat gtgggattcc tgtccaagta gctctcgatg
     5461 cccaccataa tgcgctccag caggttggcc aggtgcgggt ctagctgcgg cggattcgtc
     5521 gtctggttca ctgagtttag ggtctgcaga aagaagtgtg cctccatggt aagcggattc
     5581 atctgcacga tcagcgggct ttagtctaaa aattcgaatg atacaaactc gtactcacgt
     5641 aaagcagttt gtttggcatc atctcggcca tcgtcttatt cttcttcgtg gcctttgtta
     5701 ccggagcggc tatgttgggc gcggtggagc ccggaagctg aaaggttaat cgaatgcgtt
     5761 aacttttctg caactcgaaa gtttgccgcc tttgttcgac tgccaataac tgttgattcg
     5821 aaaattcgaa tcgaagcggt tgaatttcgt agggtggcca actacaccaa agttcgccgg
     5881 cggtgtatgc atgtgagtgt gagtgtgaca gtgggtgtac gtgtgcttta agcgacacaa
     5941 tcgcaccgcg atgtacatat ttttgtagaa atgtgtgtgc atttttcgtc cggatcacgg
     6001 ctccataaaa taaatcatat cacagggctc agggatttgt gagtagaagt tcccgccact
     6061 taaacgtcac ttgtgaaatc gccatcgacg gtcaactttt actcacactg gtcttccgcg
     6121 gacggaccgt ctcaaagtac tgcctttctg cgttggaaaa catcgccttt ttcgtccaaa
     6181 aggagtcccc aggttcgatc cgcatggcgt tgtgcgtgcg tgcctttctt ttcaaatgat
     6241 tacggctatt aacttggggg cgttaagttg gaaacacgta aattgcagac tgcgattaga
     6301 gtgaccatga gtaggagttc aaaatctcct gacatcattt tcttaaaacc tgctttgttt
     6361 tttacatttc tatttaatat aactcctatt tgaataaaaa aacaaaacaa gtttagatgt
     6421 taagatatta actacatcct ttgctccaaa gggagagggg aagttatgga gttaattaat
     6481 ttgctgttgg aaatcaatat ggagtcagaa atataatgat ttactaaacc ttattgaatc
     6541 ggtaacgatg cgaatttata ttaaaatagc ttttatgaaa cattcaacaa aaatattatt
     6601 aatgttggcc cactttagca accggttagg tctaccggtt gggcaagcaa agattcacgc
     6661 cctggttcga gtcccaacta gtcctgcaaa ataccgcagc aagttttaga gagaccaagt
     6721 gccattacct ctcccacttc agttatcggt tatgcggcgt ttaagtcgac agcttgccgt
     6781 ctctagctcc ggtgcctata taaagcagcc cgctttccac atttcatatt cgttttacgt
     6841 ttgtcaagcc tcatagccgg cagttcgaac gtatacgctc tctgagtcag acctcgaaat
     6901 cgtagctcta cacaattctg tgaattttcc ttgtcgcgtg tgaaacactt ccaataaaaa
     6961 ctcctgcagg tgagtacttt aaaaaaaaat ctagtgaaat aatgctgaaa agaaatttgt
     7021 gtgggcaaaa ttcaatgggc aaaaacgcga tgcggctttt tctcaaaatg gcggccggcc
     7081 tgcgtttttt cctcaaaagt gatgacgtca tgcctgtttt tttttttttg ttcgcaatga
     7141 ggaatggctc ttaaaatcta ctagataaaa aaaatattca ttatttctat gctgctggaa
     7201 cgcttcatta atcttaaaaa ttctaaattc ggttaccatg atacttcgac gcataactgt
     7261 agattttgga tagaattaaa gagaaaatgg cgagagagta aaattccggc gtcggcaaag
     7321 tagagcaaaa aaatcagtat accatttagc tacctctctc actcgcacgc agtgccggct
     7381 caagttgggc gcggctctgc aattatcgat tttcttgggg tgtgtaacta atcatccgtt
     7441 ttcccttcct cctcatccac agcgtgaagg tacccgcccg gggatcagat ccgcggccgc
     7501 aaaaggtagg ttcaaccact gatgcctagg cacaccgaaa cgactaaccc taattcttat
     7561 cctttacttc aggcggccgc ggctcgaggg taccaactta aaaaaaaaaa tcaaaatgaa
     7621 cactattcca gcgcgtcacg ttggagatgt ggcagctcgt gacccactcc ccctgccaca
     7681 tataagttcc agtgtggcca gtggcatgaa acgatcgttt gccactatgg ccatgctcta
     7741 taatgatacg ggcaatagca atgatgtcgg agcgcatgcg cgacgcccac cgcgcactct
     7801 gagtaacagc cgcagcacat cggctcaccg ggtgccattg ggtagctgga gcgccccgaa
     7861 ctcgccaccg cgacgcgcac tcccacacca ccccataaca gcttcgttcg acccggacgc
     7921 ctcgatcgtg attgccggaa tccgcggtgc tggaaagtcc actctcgcca taatggcttc
     7981 gacggcgatg aagcgcaaga tcgttgacct cgaatcggaa tttcaccacc tgactggcct
     8041 gtcctccagc agctacaaga aaacacacgg cccggtggac tatggtcgcc gccagatagc
     8101 aatcctgcag aacatcctta acctgcaccg aacgcgcgcc attctggtat gttcctggtt
     8161 ggagcgcgat gtgcaggcaa tgcttcagga tttttcggtc agcaacccgg tgatctatgt
     8221 cctcagggat gccaaagcca ttgaggccca cctgaaaggt tatgacaagt cgaaggtggg
     8281 tacgttgctg gatgcgacct cgaccgtcct gaggcgatgc acgcgatttg agttcttcaa
     8341 tgtctccgaa gaaaatctgg atactcattc cgccagcacg tcccccccag cggttccaga
     8401 tcagaggcat actgctccct acctgactct taagcgcgca gagcgtcact ttcttaagtt
     8461 cctgtccttg attttgccca aaggaaccat cccgttcgta gaatccgcct tcccactggc
     8521 gtcggttccg gtggagcaac gccgcttcac ctatgcgctg gcgcttcccg tgtcggcact
     8581 cctggacaag ggagtcgaca ttcaggagct tgacgtaggc gtcgacgcaa tcgaaattat
     8641 cgtagacgat ctggccacaa gcgaatccgg cccaactagc cccctcggcc tcgccccgca
     8701 ccgagcaagc gaaatctcca gggtggttgg cgagatccgc agggataccg tcatacccat
     8761 catcctccac gtggtcttcc cggagagggc cctgtacgaa gaggccctgt tggccctgta
     8821 catgacctac ctgaaccacg ccttgcgtct cgcgcccgat taccttacag tggacctcgg
     8881 actggatagc ggcctgctgg gtcagttgac gacagtgcag ggcacaacta aagtgattgg
     8941 taacaagcag ctggctgaag tgaatagccc tcgctggggc gacccatcgt ggttgcaggc
     9001 ctacgaaaag gcccaaaata ctggctgtga ccttgtgcgg ttgacacgac ccgcctcgaa
     9061 cccgcgtgac aatactgaca tccgtcagtt ccatgttgcc gtggaggctg tgggtggccc
     9121 acgactgccc ttcatcgcct ataatactgg taggctcggc cgaaccagta tgtgcttcaa
     9181 cgaaatcctg acgccagtca ctcccgtccc caccaaggag gatgccattg gtcttcgcaa
     9241 tccggcacac cgttacctgc agccaccgtt gactgcactg gaagcaaccc aggccctgta
     9301 cagcgcgttt gttcacgacc caatgaagct gtacgttttc ggagctaatg ttggatacag
     9361 cctgagcccc gctatgcaca atgccgcgtt gaaagcctgc ggaatcccgc atcattacaa
     9421 gcccctgagt actgctaaca taggcaccct tcgtgaggtg atctccgacc cgcagttcgc
     9481 gggagctagt gtaggtctgc cctttaaggt ggaaatcatt agcctgactc actcgctgag
     9541 ccgtcacgct aaagccattg gcgcggtgaa tacgttgatt cctgtgaggc atcttacagc
     9601 agatggaggc atccccgacg aggtgtccat gtttaataac atcagccagg caggtgctgt
     9661 gagggcattg tacggcgaga ataccgattg gatcggaatc cgtgcctgtc tgcgtcgagg
     9721 tttgagtcct gcaaatgcag tccgctccac atccactgga ttggtgatcg gagcaggcgg
     9781 catggcccga gcagcggtgt acgcaatgct ccagctcgga gtcaagaaga tcctcatttt
     9841 caaccgcacc ttcgctaacg ccgagaagct cgtgctgcat ttcgagaatc tgttggtcag
     9901 ggatgccctg cccctgttgt cgacaggccc tcgcagccac gataacacgt gttttcacat
     9961 catacggtcg agggatgatc cgctcccaga gaacttcaag aaccctacca tgatagtgtc
    10021 gtgcatccca acgcacactg tagataatac gcccgatccc gagttcaccg tgccattgca
    10081 ctggttggac aacccgaccg gcggaatcgt actggagctg gactacaagt gcttgacaag
    10141 tccactgttg gagcagacgc ggcgtgaggc tcatcgcgga tgggttgcga tggatggctt
    10201 ggacctgctc cctgaacaag gatttgcgca gtttgaattg ttcaccggcc gacgtgcacc
    10261 gcgtcgcctg atgcgccgcg aggtcctgcg agcatatccg gacgaccagg ccaagagtca
    10321 tactgcacag ctgcaacctc gtctcaatgg aatagcaacg cagatttcgt aatctagaat
    10381 gaatcgtttt taaaataaca aatcaattgt tttataatat tcgtacgatt ctttgattat
    10441 gtaataaaat gtgatcatta ggaagattac gaaaaatata aaaaatatga gttctgtgtg
    10501 tataacaaat gctgtaaacg ccacaattgt gtttgttgca aataaaccca tgattatttg
    10561 attaaaattg ttgttttctt tgttcataga caatagtgtg ttttgcctaa acgtgtactg
    10621 cataaactcc atgcgagtgt atagcgagct agtggctaac gcttgcccca ccaaagtaga
    10681 ttcgtcaaaa tcctcaattt catcaccctc ctccaagttt aacatttggc cgtcggaatt
    10741 aacttctaaa gatgccacat aatctaataa atgaaataga gattcaaacg tggcgtcatc
    10801 gtccgtttcg accatttccg aaaagaactc gggcataaac tctatgattt ctctggacgt
    10861 ggtgttgtcg aaactctcaa agtacgcagt caggaacgtg cgcgacatgt cgtcgggaaa
    10921 ctcgcgcgga aacatgttgt tgtaaccgaa cgggtcccat agcgccaaaa ccaaatctgc
    10981 cagcgtcaat agaatgagca cgatgccgac aatggagctg gcttggatag cgattcgatc
    11041 gagttaacat cgaggtcgac ctgcagccaa gctttgcgta ctcgcaaatt attaaaaata
    11101 aaactttaaa aataatttcg tctaattaat attatgagtt aattcaaacc ccacggacat
    11161 gctaagggtt aatcaacaat catatcgctg tctcactcag actcaatacg acactcagaa
    11221 tactattcct ttcactcgca cttattgcaa gcatacgtta agtggatgtc tcttgccgac
    11281 gggaccacct tatgttattt catcatggtc tggccattct catcgtgagc ttccgggtgc
    11341 tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa
    11401 ataactgctt gttttttttt ttaccattat taccatcgtg tttactgttt attgccccct
    11461 caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct atagaataca
    11521 agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct aaccaaaaga
    11581 cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat
    11641 atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac
    11701 accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct
    11761 tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtg cggtatttca
    11821 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
    11881 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
    11941 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
    12001 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
    12061 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
    12121 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    12181 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
    12241 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
    12301 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
    12361 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
    12421 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
    12481 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    12541 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    12601 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    12661 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    12721 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    12781 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    12841 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    12901 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    12961 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    13021 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    13081 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    13141 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    13201 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    13261 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    13321 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    13381 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    13441 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    13501 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    13561 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    13621 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    13681 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    13741 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    13801 tgatgctcgt caggggggcg gagcctatgg aaaaacgcct tcttcttgaa ctcgggctcg
    13861 gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt
    13921 ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac
    13981 accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat
    14041 ctgccgctgg actacgtggg tctggcc
//
