LOCUS       sequence-65108-e         963 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A
  TITLE     Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end 
            rule pathway.
  JOURNAL   J Biol Chem. 2008 Aug 29. 283(35):24011-28.
  PUBMED    18566452
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Aug 29. 283(35):24011-28."
COMMENT     Sequence Label: UPR pBARUra3
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(217..243)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(382..609)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(637..663)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc
       61 aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg
      121 cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg
      181 ttccttatat gtagctttcg actggccaag gaaagcggcg taatctggga cgtcgtatgg
      241 gtagccacgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      301 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      361 ccaagctccg gatccgtgtc taccacctct tagccttagc acaagatgta aggtggactc
      421 cttctgaatg ttgtaatcag acagcgttct accgtcttct agctgcctac cggcaaagat
      481 caatctttgt tgatctggag ggataccttc cttgtcttga attttcgact taacgttgtc
      541 gatggtatcg gaagattcaa cttccaatgt tatggtttta ccggtcaaag tcttgacgaa
      601 aatctgcatg gtcgacccac tacccgcaag gaaagcggcg taatctggga catcgtatgg
      661 gtaggtaccg tctttcttct cgtagacttc aaacttatac ttgatgcctt tttcctcctg
      721 gacctcagag aggacgcctg ggtattctgg gagaagttta tatttcccca aatcaatttc
      781 tgggaaaaac gtgtcacttt caaattcctg catgatcctt gtcacaaaga gtctgaggtg
      841 gccctggttg attcatgggc ttcctgggta aacagaactg cctccgacta tccaaaccca
      901 tgtctacttt acttgccaat tccggttgtt caataagtct taaggcatca tccaaacttt
      961 tgg
//
