LOCUS       sequence-65404-e        4224 bp    DNA     circular SYN 14-APR-2025
DEFINITION  For expression of C-terminal fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Kitagawa D, Vakonakis I, Olieric N, Hilbert M, Keller D, Olieric V, 
            Bortfeld M, Erat MC, Fluckiger I, Gonczy P, Steinmetz MO
  TITLE     Structural basis of the 9-fold symmetry of centrioles.
  JOURNAL   Cell. 2011 Feb 4;144(3):364-75. doi: 10.1016/j.cell.2011.01.008.
  PUBMED    21277013
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2011.01"; journalName: "Cell"; date: "2011-02-4- 4";
            volume: "144"; issue: "3"; pages: "364-75"
COMMENT     Sequence Label: pENTR SD Age HsSAS-6
FEATURES             Location/Qualifiers
     source          1..4224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2453..2552)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2664..2683)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2675..3484
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2742..2761)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3574..4162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4063..4082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccgg taccatgagc
      481 caagtgctgt tccaccaact agtcccgttg caggtgaaat gcaaagactg tgaggagagg
      541 agagtaagta taagaatgag cattgaacta caatcagttt ctaatccagt tcacagaaag
      601 gacttagtta ttcgtctgac tgatgacacg gatccatttt ttttatataa ccttgttata
      661 tctgaggaag attttcaaag tttaaaattc cagcaaggtc ttctggtaga cttcttagct
      721 ttcccacaaa aatttataga tctccttcag caatgtactc aagaacatgc caaagaaatt
      781 ccaaggtttt tgctacagtt agtttctcca gcagctattt tggataactc acctgcattt
      841 ttaaatgtgg tagagacaaa tccttttaag catcttacac acctctcact aaaactttta
      901 cctggaaatg atgtggagat aaagaaattt ctcgcaggct gtttgaaatg tagcaaggaa
      961 gaaaaattat cattgatgca atcactagat gatgctacta agcaactgga ctttacacga
     1021 aagacattag cagaaaaaaa acaagagctc gataagttac ggaatgaatg ggcgtcacat
     1081 acagcagcct tgacaaacaa gcattctcag gaactgacaa atgaaaagga aaaagccttg
     1141 caggcacagg ttcaatatca acagcagcat gaacaacaga aaaaagattt agaaatcctc
     1201 catcaacaaa acatccacca gctacaaaac agactgtctg agttagaagc ggctaataaa
     1261 gacttaaccg aaagaaaata taaaggagac tccactatta gagaacttaa agcaaaactt
     1321 tctggtgttg aagaggagct acagcggact aagcaagaag tcctctcttt gcgaagagag
     1381 aattctacac tagatgttga atgccacgag aaagaaaagc acgttaatca gctacaaaca
     1441 aaagtggcag ttttagaaca ggaaatcaag gataaggacc agcttgtttt aagaacaaaa
     1501 gaggcatttg atacaatcca ggaacaaaag gtggttttag aagaaaatgg tgagaaaaat
     1561 caagtacaac taggaaagct tgaagctaca ataaaatcat tatctgcaga acttctgaag
     1621 gcaaatgaaa ttatcaagaa gttacaaggg gatctgaaaa ctttaatggg taagttgaaa
     1681 ttgaagaata cagttactat tcagcaagaa aaactcttgg ctgagaagga ggaaaaatta
     1741 caaaaggaac aaaaggaatt acaagatgtt ggacagtctc ttcgaattaa agagcaagag
     1801 gtatgcaaat tacaagaaca attagaagct acagttaaaa aacttgaaga aagcaaacaa
     1861 cttctaaaaa ataatgaaaa gttaatcacg tggttaaata aagaactaaa tgaaaatcag
     1921 ctagtgagaa agcaagatgt attgggacct tctactactc cgcctgcaca ttccagcagc
     1981 aacacaatca gaagtggaat ttctcctaac ctgaatgtgg ttgatggtag actgacttac
     2041 ccaacctgtg ggattggtta tcctgtctcc tctgcatttg cattccagaa taccttccct
     2101 cattcgatat ctgccaaaaa taccagccac cctggttcag gaacaaaggt tcagtttaat
     2161 ttgcagttta caaaaccaaa tgcatcacta ggagatgttc agtcaggagc aactattagt
     2221 atgccttgct caactgataa ggaaaatggt gaaaatgtag ggttggaatc caaatacctg
     2281 aagaaaaggg aagatagcat tcctttacgc ggactcagcc agaacctatt tagtaattca
     2341 gaccatcaga gagatggcac tttaggagca ttacatacat cttccaaacc cacagcgctc
     2401 ccctctgcgt cttcagccta tttccctggg cagttaccaa acagttatct agacccagct
     2461 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     2521 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc
     2581 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     2641 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     2701 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     2761 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     2821 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     2881 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     2941 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     3001 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgtccc tgcgccggtt
     3061 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     3121 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     3181 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     3241 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     3301 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     3361 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     3421 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     3481 ctaatcagaa ttggttaatt ggttgtaaca ttattcagat tgggccccgt tccactgagc
     3541 gtcagacccg gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3601 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3661 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3721 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3781 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3901 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3961 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4021 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4081 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4141 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4201 ttgctggcct tttgctcaca tgtt
//
