LOCUS       sequence-65874-e        8133 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.U6.miR-1306-Decoy.hPGK.GFP.WPRE
FEATURES             Location/Qualifiers
     source          1..8133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(32..100,101..892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(250..269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1063..1651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1552..1571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1805..1822
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1939..1960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1975..1980,1981..1998,1999..2005)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2013..2029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2018..2040
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2037..2053
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2037..2053
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2072..2092
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2074..2092
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2193..2572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2573..2776
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2727..2747
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2791..2971
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3015..3140
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3633..3866
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4051..4095
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4362..4479
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(4582..4894)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(4643..4663)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(4896..4912)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4896..4912)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        4932..5442
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5361..5379
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      5458..5467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5464..5466,5467..5469,5470..6183)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5464..5466,5467..5469,5470..6180)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5509..5530)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5770..5789)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6117..6138
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6199..6787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6252..6272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6670..6681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6874..7107
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7179..7300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7216..7235)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7270..7289
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7319..7454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7486..7505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7487..7505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7515..7532)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7515..7531)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7524..7546)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7676..8131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7763..7782)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7973..7994
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
       61 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      121 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      181 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      241 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      301 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      361 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      421 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      481 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      541 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      601 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      661 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      721 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      781 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
      841 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
      901 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
      961 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1021 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1081 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1141 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1201 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1261 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1321 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1381 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1441 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1501 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1561 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1621 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1681 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1741 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1801 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     1861 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     1921 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     1981 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     2041 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     2101 gctggagctg caagcttggc cattgcatac gttgtatcca tatcataata tgtacattta
     2161 tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata
     2221 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2281 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2341 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2401 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2461 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2521 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     2581 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     2641 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     2701 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     2761 ctatataagc agagctcgtt tagtgaaccg gggtctctct ggttagacca gatctgagcc
     2821 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     2881 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     2941 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga
     3001 aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     3061 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     3121 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     3181 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     3241 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     3301 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     3361 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     3421 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     3481 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     3541 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     3601 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     3661 gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta
     3721 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     3781 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     3841 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     3901 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     3961 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     4021 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     4081 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     4141 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     4201 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     4261 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     4321 tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg
     4381 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     4441 aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga gactagcctc
     4501 gagaagcttg atatcgaatt caaaaaagac ggcgctagga tcatcgagac ggtaccgtct
     4561 ccgatgatcc tagcgccgtc aaacaaggct tttctccaag ggatatttat agtctcaaaa
     4621 cacacaatta ctttacagtt agggtgagtt tccttttgtg ctgtttttta aaataataat
     4681 ttagtatttg tatctcttat agaaatccaa gcctatcatg taaaatgtag ctagtattaa
     4741 aaagaacaga ttatctgtct tttatcgcac attaagcctc tatagttact aggaaatatt
     4801 atatgcaaat taaccggggc aggggagtag ccgagcttct cccacaagtc tgtgcgaggg
     4861 ggccggcgcg ggcctagaga tggcggcgtc ggatggatcc actagttcta gagcggccat
     4921 cgaattccca cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga
     4981 cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca
     5041 ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg
     5101 gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg
     5161 acgtgacaaa cggaagccgc acgactcact agtaccctcg cagacggaca gcgccaggga
     5221 gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcggggcgc
     5281 gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg
     5341 gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca
     5401 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc accggtcgcc
     5461 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     5521 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     5581 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5641 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     5701 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5761 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5821 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5881 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     5941 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     6001 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     6061 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     6121 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     6181 taaagcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6241 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6301 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6361 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6421 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6481 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6541 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6601 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6661 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6721 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6781 tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     6841 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6901 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6961 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7021 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7081 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     7141 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7201 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7261 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7381 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7441 cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg
     7501 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7561 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7621 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg
     7681 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7741 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7801 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7861 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7921 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7981 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8041 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8101 aattttaaca aaatattaac gtttacaatt tcc
//
