LOCUS       sequence-66346-e         858 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX303
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..152
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(177..182,183..200,201..207)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(327..346)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gagctctctg gctaactgtc gggatcaaca agtttgtaca aaaaagctga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
      181 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
      241 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
      301 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      361 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      421 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
      481 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      541 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      601 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      661 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
      721 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
      781 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
      841 ccgtttgtga tggcttcc
//
