LOCUS       sequence-66380-e        7567 bp    DNA     circular SYN 15-APR-2025
DEFINITION  A retroviral vector for the constitutive expression of soluble 
            guanylate cyclase1 beta3 double mutant (E473K and C541D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Ochoa CD, Alexeyev M, Pastukh V, Balczon R, Stevens T
  TITLE     Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that 
            increases endothelial tau phosphorylation and permeability.
  JOURNAL   J Biol Chem. 2012 Jul 20;287(30):25407-18. doi: 
            10.1074/jbc.M111.301440. Epub 2012 May 25.
  PUBMED    22637478
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.301440"; journalName: "J Biol Chem"; date: 
            "2012-07-20- 20"; volume: "287"; issue: "30"; pages: "25407-18"
COMMENT     Sequence Label: pMA3411
FEATURES             Location/Qualifiers
     source          1..7567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3295..3311)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3295..3311)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     3368..3384
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3369..3385
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3407..3427)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3412..3741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3592..3727
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3773..4795
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             4844..5437
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5638..5655)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5809..6397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5889..5908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6579..7370,7371..7439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7202..7221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7440..7544)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttagat cactggaaag atgtcgagcg gctcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcttcc atccgcgccg tctctccccc ttgaacctcc tctttcgacc ccgcctcaat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatcaat tcgccagcac
     1381 agtggtcgaa tcgaattcgc caccatgtac ggttttgtga accatgccct ggagctgctg
     1441 gtgatccgca attacggtcc cgaggtgtgg gaagacatca aaaaagaggc gcagctggat
     1501 gaagaaggcc agtttcttgt gagaataatc tacgatgatt ccaaaaccta tgacttggtg
     1561 gctgctgcga gcaaagtcct caacctcaat gctggtgaaa tcctgcagat gtttgggaag
     1621 atgtttttcg tcttctgtca agagtctggc tatgatacca tcttgcgtgt cctgggatct
     1681 aatgtcaggg agtttttgca gaacctcgac gccctgcacg accacctcgc caccatctac
     1741 ccagggatgc gcgcaccttc cttccggtgc accgatgcag aaaaaggcaa agggctcatt
     1801 ctgcactact actcggaaag agaggggctt caggacattg tgatcgggat tatcaagact
     1861 gtagctcaac agatccatgg cactgagata gacatgaagg ttattcagca aagaagtgaa
     1921 gaatgtgatc atacccaatt tttaattgaa gaaaaagaat caaaagaaga ggatttttat
     1981 gaagatctgg acaggtttga agagaacggt acccaggact cccgtatcag cccgtacacc
     2041 ttctgcaaag cgtttccttt tcacatcata tttgaccggg acctagtagt cacgcagtgt
     2101 ggaaatgcta tctacagagt gctcccccag ctccagcctg ggaagtgcag ccttctgtct
     2161 gtcttctctc tggtccgccc tcatattgac atcagtttcc acgggattct ttcacacatc
     2221 aataccgtct ttgtactgag aagcaaggaa gggttgctgg atgttgagaa acttgaatgt
     2281 gaggatgaac tgactggggc agagattagc tgcctccgtc tcaaaggcca aatgatctat
     2341 ttaccggaag cagatagcat cctcttcctc tgttcaccaa gtgtgatgaa cttggatgac
     2401 ctaacaagaa gaggcctgta cctgagtgac atccctctcc atgatgctac acgagacctg
     2461 gtccttttgg gagaacagtt ccgggaggag tacaaactga cacaagagct ggaaatcctc
     2521 acagacaggc tgcagctcac actgagggct ttggaggatg agaagaaaaa gacagacaca
     2581 ttgctatatt ctgttctccc tccatctgtt gccaatgagc tgagacacaa gcgtccagtg
     2641 ccggccaaaa gatacgacaa tgtgaccatc ctcttcagtg gcattgtggg cttcaacgct
     2701 ttctgtagca agcatgcatc tggagaaggg gccatgaaga ttgtcaatct actcaacgac
     2761 ctctacacca gattcgacac actgactgat tcacggaaaa acccatttgt ttacaaggtg
     2821 aaaacagttg gtgacaagta tatgacagtg agtggtttgc cagaaccttg tatccaccat
     2881 gcacggtcca tttgccacct ggctttagac atgatggaaa ttgctggtca agttcaagta
     2941 gatggcgaat ctgttcagat aaccatcggg atccacactg gggaggtggt gacaggtgtg
     3001 attggacagc ggatgccacg gtatgatctc ttcggaaata ctgtcaacct cacaagcagg
     3061 acagaaacca caggagaaaa ggggaagata aacgtttccg aatatacata caggtgtctc
     3121 atgtccccag aaaactcgga tccacagttc cacttggagc acagaggtcc tgtgtccatg
     3181 aagggcaaga aggagccaat gcaagtctgg tttctatcca ggaaaaacac aggcacagag
     3241 gaaacaaacc aggatgaaaa ctgaatctag agatcgaatt cctgcagccc gggggatcca
     3301 ctagttctag agcggccgcc accgcggtgg agctcgaatt gacgcgtcct cggtaccggg
     3361 ccccccctcg aggtcgacgg tatcgataag cttgattcga ccctgtggaa tgtgtgtcag
     3421 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3481 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3541 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3601 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3661 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3721 ggaggcctag gcttttgcaa aaagctagct taccatgacc gagtacaagc ccatgaaaaa
     3781 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     3841 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     3901 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     3961 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt
     4021 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     4081 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     4141 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     4201 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     4261 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     4321 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     4381 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     4441 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     4501 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     4561 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     4621 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     4681 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     4741 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatatcg
     4801 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     4861 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
     4921 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
     4981 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
     5041 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     5101 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
     5161 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     5221 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     5281 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
     5341 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     5401 tgagtgattg actacccgtc agcgggggtc tttcatttcc gacttgtggt ctcgctgcct
     5461 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtcttcac atgcagcatg
     5521 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt
     5581 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggcgctctt ccgcttcctc
     5641 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5701 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5761 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5821 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5881 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5941 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6001 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6061 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6121 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6181 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6241 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6301 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6361 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6421 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6481 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agtttgcggc cggccgcaaa
     6541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6721 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7561 gtgccac
//
