LOCUS       sequence-66695-e        9389 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Retroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged 
            AP-1 gamma subunit and G418 resistance gene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Robinson MS, Sahlender DA, Foster SD
  TITLE     Rapid inactivation of proteins by rapamycin-induced rerouting to 
            mitochondria.
  JOURNAL   Dev Cell. 2010 Feb 16;18(2):324-31. doi: 
            10.1016/j.devcel.2009.12.015.
  PUBMED    20159602
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2009.12"; journalName: "Dev Cell"; date: 
            "2010-02-16- 16"; volume: "18"; issue: "2"; pages: "324-31"
COMMENT     Sequence Label: pLXIN-Ap1g1-FKBP
FEATURES             Location/Qualifiers
     source          1..9389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             join(3818..3820,3821..4141)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     misc_feature    4823..5396
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4846..5398
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4990..5007)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5217..5236
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5398..6201
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5461..5480)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6071..6090
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6261..6854
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6957..6979)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7065..7205
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7120..7139
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7220..7237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7391..7979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7471..7490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8150..8941,8942..9010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8773..8792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9011..9115)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9183..9201
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aattccgcag tcgcctcctc ctcggcaaat
     1501 agtcctcggc gggggccgag tcctcgggtc atccggggtg ggagacggca cgggctgcag
     1561 tcggagggac gcggggcggc cggcgagagg gagccgggga gcggcgtctc ccggggaagc
     1621 gcgaggcgtg aggtagaggc gccctcctcg cccgggatcc ctccgcctgc agtcgcggtg
     1681 ctgaggccga gggagccgcc attttggatg ttcggttcct gctgccactg ccgccgccgc
     1741 ccccgaagct gctggtttcc ttcgaggttt cgggccgagg atgccagccc ccatcagatt
     1801 gcgggagctg atccggacca tccggacagc ccgaacccaa gctgaggaac gagaaatgat
     1861 ccagaaagaa tgtgctgcaa tccggtcctc atttagagaa gaagacaata cataccggtg
     1921 tcggaatgtg gcaaagttac tatatatgca catgctgggc taccctgctc actttggaca
     1981 gttggagtgc ctcaagctaa tcgcctcaca aaaattcaca gacaaacgca ttggctattt
     2041 aggagcaatg ctattattag atgaaagaca agatgtgcat cttctcatga ccaactgtat
     2101 caagaatgat cttaatcata gcacgcagtt tgtgcagggg ctagcacttt gtaccctcgg
     2161 ctgcatgggc tcctcggaga tgtgcagaga tcttgcggga gaggtagaaa agcttctgaa
     2221 aacctccaac tcttacttaa gaaaaaaggc agcactgtgt gctgttcacg tcatcaggaa
     2281 ggttcctgaa cttatggaga tgtttttacc agcaacaaaa aatttattga atgagaagaa
     2341 ccatggtgtc cttcacacgt ctgtagtcct cctcacagag atgtgtgaac ggagcccaga
     2401 catgctcgca catttcagaa agcttgtgcc ccaattagtt cgtattctaa agaacctcat
     2461 catgtccgga tattccccag aacatgatgt ctctggtatc agtgacccct ttttgcaggt
     2521 acgaattttg cggttattaa gaattttagg acggaatgac gatgattcaa gtgaagctat
     2581 gaatgatata ttagcacagg ttgccactaa tacagagact agtaaaaatg taggaaatgc
     2641 tattctgtat gagacggttt tgactatcat ggatattaaa tcagagagtg gattgcgagt
     2701 tctagccata aatattcttg gtcgattctt attgaacaat gacaaaaata ttagatatgt
     2761 tgctctgaca tctctgttga agactgtgca gacagatcat aacgctgtac agaggcatag
     2821 aagcacaatt gtggactgtc tgaaagatct ggatgtctcc ataaaaagac gtgcaatgga
     2881 actgagtttt gccctggtga atgggaataa tatccgaggc atgatgaaag aattacttta
     2941 ttttctggat tcctgtgagc cagaatttaa agctgattgt gcatctggaa tcttccttgc
     3001 tgcagaaaag tacgcacctt ccaaacggtg gcatatagat acaattatgc gtgtcttgac
     3061 aacggcagga agttatgttc gcgatgatgc agttcctaac ttgattcagt taataaccaa
     3121 cagtgtggag atgcacgctt acaccgtcca gcgcctgtac aaggcgattc tcggtgacta
     3181 ttctcaacaa cccttggtac aagtggctgc gtggtgtata ggtgaatatg gcgatcttct
     3241 tgtgtctggc cagtgtgaag aggaagagcc tattcaggtg acagaagatg aagtgctgga
     3301 tattttagaa agtgtcctga tctctaatat gtccacctct gtgacaagag gctatgctct
     3361 cactgccatt atgaagctgt ctacccgatt cacctgtact gtaaaccgaa ttaagaaagt
     3421 ggtttccatc tatgggagca gcatcgacgt agagctccag cagagggcag tagagtacaa
     3481 cgcacttttt aagaagtatg accacatgag gtctgcccta cttgaaagaa tgcctgtcat
     3541 ggaaaaagtg accacaaatg gcccttcgga gatcgtgcag acaaatggag agacagaacc
     3601 agcgccccta gagactaaac caccaccctc agggccacaa cccacaagcc aggccaatga
     3661 tctattggat ttgttgggag gaaatgacat aacacctgtt atcccaactg cacctacaag
     3721 caaaccagca tcagctggtg gagaacttct cgacttgcta ggagacatca ccatcgatgt
     3781 gaatgggggc cctgaacccg ccaagaacct gtacaccatg ggagtgcagg tggaaaccat
     3841 ctccccagga gacgggcgca ccttccccaa gcgcggccag acctgcgtgg tgcactacac
     3901 cgggatgctt gaagatggaa agaaatttga ttcctcccgg gacagaaaca agccctttaa
     3961 gtttatgcta ggcaagcagg aggtgatccg aggctgggaa gaaggggttg cccagatgag
     4021 tgtgggtcag agagccaaac tgactatatc tccagattat gcctatggtg ccactgggca
     4081 cccaggcatc atcccaccac atgccactct cgtcttcgat gtggagcttc taaaactgga
     4141 aggatccatc gatctgacag agctggagcc ccctgcccct gagagcccca tggctttgtt
     4201 ggctgaccca gctccagctg ctgaccccgg ggctccagcg gccgctccta cccctgcctc
     4261 agtgccacag atatcccagc cccccttctt gttggatggg ctttcctcac agcctctctt
     4321 caatgacatc gctccaggca tcccctccat cacagcgtac agtaagaacg gcttgaaaat
     4381 cgagtttaca tttgaacggt caaacaccaa ccccagcgta acagtgataa cgatacaggc
     4441 ctctaacagc acagagctag acatgacgga ctttgttttc caggctgcag taccaaagac
     4501 attccagctg cagcttctgt ctcctagcag cagcgttgtc ccagccttta atacagggac
     4561 catcacacaa gtcattaaag ttctgaatcc acagaagcaa cagctgcgaa tgcggatcaa
     4621 gctcacatat aatcacaagg gctcggcaat gcaagatcta gcagaagtga acaatttccc
     4681 ccctcagtcc tggcaatgag gatgcagcac cattctcatt cttatcccac tcaatcaaag
     4741 gaattcgtta acgtcgacct cgagggatcc gcggccgcaa attcttaacg tcgacctcga
     4801 gggatccgcg gccgcaaatt cgcccctctc cctccccccc ccctaacgtt actggccgaa
     4861 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtg attttccacc atattgccgt
     4921 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     4981 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     5041 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     5101 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     5161 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     5221 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     5281 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     5341 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     5401 ggatcggcca ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5461 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5521 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5581 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5641 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5701 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5761 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5821 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5881 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     5941 cccgacggcg atgatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     6001 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6061 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6121 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6181 cttcttgacg agttcttctg agcgggactc tggggttcga taaaataaaa gattttattt
     6241 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     6301 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     6361 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     6421 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
     6481 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     6541 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     6601 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     6661 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     6721 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     6781 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     6841 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
     6901 cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6961 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7021 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     7081 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     7141 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     7201 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7261 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7321 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7381 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7441 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7501 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7561 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7621 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7681 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7741 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7801 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7861 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7921 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7981 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8041 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8101 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8161 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8221 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8281 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8341 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8401 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8461 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8521 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8581 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8641 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8701 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8761 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8821 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8881 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8941 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9001 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9061 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9121 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     9181 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt gctagcaatt gctagcaatt
     9241 gctagcaatt cataccagat caccgaaaac tgtcctccaa atgtgtcccc ctcacactcc
     9301 caaattcgcg ggcttctgcc tcttagacca ctctacccta ttccccacac tcaccggagc
     9361 caaagccgcg gcccttccgt ttctttgct
//
