LOCUS       sequence-66901-e         842 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Yoo SK, Deng Q, Cavnar PJ, Wu YI, Hahn KM, Huttenlocher A
  TITLE     Differential regulation of protrusion and polarity by PI3K during 
            neutrophil motility in live zebrafish.
  JOURNAL   Dev Cell. 2010 Feb 16. 18(2):226-36.
  PUBMED    20159593
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Feb 16. 18(2):226-36."
COMMENT     Sequence Label: Tol2-mpx:Lifeact-Ruby
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             728..745
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             complement(759..809)
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      806..815
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atacgactca ctatagttct agatcagtcg acttaagcgc ctgtgctatg tctgccctca
       61 gctctctcat attgttccac gatggtgtag tccccattat ggctgataat gtcgagttta
      121 atatcagtca tgtaggcgcc aggcagctgc acttgtttct tggccttgta ggttgttttg
      181 acctcggcat cgtagtgtcc tccgtctttg agtttcagtc tcattttaat ttcgcctttc
      241 agagcgccat cctcaggata cattctctcg gtggaggctt cccatcccat tgtttttttt
      301 tgcatgacag ggccatcgga ggggaagttg gttcctctga gtttcacctt ataaataaac
      361 tctccgtcct ggagggtgct atcttgtgtg actgtcacca cgcctccgtc ctcgaagttc
      421 atgaatctct cccacttgaa gccttcaggg aaggagagct tcagatagtc agggatgtcg
      481 gcagggtgtt taacataggc tttgcttccg tactggaact gagggctcag aatatcccag
      541 gagaagggga ggggtcctcc ttttgtgacc ttgagcttag cggtttgtgt gccctcgtaa
      601 ggccggcctt ctccttctcc ctcaatctcg aactcgtgtc cgttgacgct tccctccatc
      661 ttgaccttaa atctcataaa ctctttgatg acatcctcgg agctggccat ggtaagcttg
      721 cccatagcac cagcaccagc accagcaccc ggatcccctt cttcctttga gatgctttcg
      781 aatttcttga tcaaatctgc gacacccatg gtggcgagat ccctaaaaac acatattgag
      841 ac
//
