LOCUS       sequence-67160-e         897 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K
  TITLE     Supersensitive Ras activation in dendrites and spines revealed by 
            two-photon fluorescence lifetime imaging.
  JOURNAL   Nat Neurosci. 2006 Feb . 9(2):283-91.
  PUBMED    16429133
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2006 Feb . 9(2):283-91."
COMMENT     Sequence Label: mEGFP-HRas
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(86..88,89..91,92..802)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(131..152)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(392..411)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     739..760
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
       61 taggctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
      121 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      181 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      241 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      301 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      361 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      421 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      481 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      541 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      601 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      661 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
      721 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      781 cggcatggac gagctgtaca agtccggact cgagatgacg gaatataagc tggtggtggt
      841 gggcgccggc ggtgtgggca agagtgcgct gaccatccag ctgatccaga accattt
//
