LOCUS       sequence-68111-e        9646 bp    DNA     circular SYN 14-APR-2025
DEFINITION  wt esaR promoter in pCS26.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 9646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pCS-PesaRlux
FEATURES             Location/Qualifiers
     source          1..9646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             312..1754
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1766..2689
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2738..3820
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3835..4818
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4997..6109
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      6142..6228
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             6341..7291
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(8510..8604)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8635..9429)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8746..8765)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9356..9375
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9608..9626
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcagattgag taaccgtgaa tgtttgtaca aatgtttcaa agatgttact atgagtgtcc
       61 cggccagcat cactttatat tttgtgacgt ctggccggac gttttcccta gtgttggctg
      121 ttttagcgac ctggccgtac aggtcaggtt tttttttacc gctaaacaac tgaagccatt
      181 gtaacctctg aatgattcat tgtaagttac tcttaagtat catcttgcct gtactatagt
      241 gcaggttaag tccacgttaa gtaaaagaag cagcggatcc tctagttgcg gccgcaaaat
      301 ggatggcaaa tatgactaaa aaaatttcat tcattattaa cggccaggtt gaaatctttc
      361 ccgaaagtga tgatttagtg caatccatta attttggtga taatagtgtt tacctgccaa
      421 tattgaatga ctctcatgta aaaaacatta ttgattgtaa tggaaataac gaattacggt
      481 tgcataacat tgtcaatttt ctctatacgg tagggcaaag atggaaaaat gaagaatact
      541 caagacgcag gacatacatt cgtgacttaa aaaaatatat gggatattca gaagaaatgg
      601 ctaagctaga ggccaattgg atatctatga ttttatgttc taaaggcggc ctttatgatg
      661 ttgtagaaaa tgaacttggt tctcgccata tcatggatga atggctacct caggatgaaa
      721 gttatgttcg ggcttttccg aaaggtaaat ctgtacatct gttggcaggt aatgttccat
      781 tatctgggat catgtctata ttacgcgcaa ttttaactaa gaatcagtgt attataaaaa
      841 catcgtcaac cgatcctttt accgctaatg cattagcgtt aagttttatt gatgtagacc
      901 ctaatcatcc gataacgcgc tctttatctg ttatatattg gccccaccaa ggtgatacat
      961 cactcgcaaa agaaattatg cgacatgcgg atgttattgt cgcttgggga gggccagatg
     1021 cgattaattg ggcggtagag catgcgccat cttatgctga tgtgattaaa tttggttcta
     1081 aaaagagtct ttgcattatc gataatcctg ttgatttgac gtccgcagcg acaggtgcgg
     1141 ctcatgatgt ttgtttttac gatcagcgag cttgtttttc tgcccaaaac atatattaca
     1201 tgggaaatca ttatgaggaa tttaagttag cgttgataga aaaacttaat ctatatgcgc
     1261 atatattacc gaatgccaaa aaagattttg atgaaaaggc ggcctattct ttagttcaaa
     1321 aagaaagctt gtttgctgga ttaaaagtag aggtggatat tcatcaacgt tggatgatta
     1381 ttgagtcaaa tgcaggtgtg gaatttaatc aaccacttgg cagatgtgtg taccttcatc
     1441 acgtcgataa tattgagcaa atattgcctt atgttcaaaa aaataagacg caaaccatat
     1501 ctatttttcc ttgggagtca tcatttaaat atcgagatgc gttagcatta aaaggtgcgg
     1561 aaaggattgt agaagcagga atgaataaca tatttcgagt tggtggatct catgacggaa
     1621 tgagaccgtt gcaacgatta gtgacatata tttctcatga aaggccatct aactatacgg
     1681 ctaaggatgt tgcggttgaa atagaacaga ctcgattcct ggaagaagat aagttccttg
     1741 tatttgtccc ataataggta aaagtatgga aaatgaatca aaatataaaa ccatcgacca
     1801 cgttatttgt gttgaaggaa ataaaaaaat tcatgtttgg gaaacgctgc cagaagaaaa
     1861 cagcccaaag agaaagaatg ccattattat tgcgtctggt tttgcccgca ggatggatca
     1921 ttttgctggt ctggcggaat atttatcgcg gaatggattt catgtgatcc gctatgattc
     1981 gcttcaccac gttggattga gttcagggac aattgatgaa tttacaatgt ctataggaaa
     2041 gcagagcttg ttagcagtgg ttgattggtt aactacacga aaaataaata acttcggtat
     2101 gttggcttca agcttatctg cgcggatagc ttatgcaagc ctatctgaaa tcaatgcttc
     2161 gtttttaatc accgcagtcg gtgttgttaa cttaagatat tctcttgaaa gagctttagg
     2221 gtttgattat ctcagtctac ccattaatga attgccggat aatctagatt ttgaaggcca
     2281 taaattgggt gctgaagtct ttgcgagaga ttgtcttgat tttggttggg aagatttagc
     2341 ttctacaatt aataacatga tgtatcttga tataccgttt attgctttta ctgcaaataa
     2401 cgataattgg gtcaagcaag atgaagttat cacattgtta tcaaatattc gtagtaatcg
     2461 atgcaagata tattctttgt taggaagttc gcatgacttg agtgaaaatt tagtggtcct
     2521 gcgcaatttt tatcaatcgg ttacgaaagc cgctatcgcg atggataatg atcatctgga
     2581 tattgatgtt gatattactg aaccgtcatt tgaacattta actattgcga cagtcaatga
     2641 acgccgaatg agaattgaga ttgaaaatca agcaatttct ctgtcttaaa atctattgag
     2701 atattctatc actcaaatag caatataagg actctctatg aaatttggaa actttttgct
     2761 tacataccaa cctccccaat tttctcaaac agaggtaatg aaacgtttgg ttaaattagg
     2821 tcgcatctct gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga
     2881 gtttggtttg cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa
     2941 attgaatgta ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga
     3001 agatgtgaat ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg
     3061 gctttacaac aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc
     3121 ggaatgctgg tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa
     3181 tgaacatatc aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc
     3241 accggtttat gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct
     3301 accgatgata ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta
     3361 taatgaagtg gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata
     3421 tataacatct gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg
     3481 gcattggtat gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag
     3541 aggttatgat ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac
     3601 taatcgccgt attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat
     3661 tgacataatt caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga
     3721 agctaatgga acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat
     3781 gccatttctt aaagaaaaac aacgttcgct attatattag ctaaggagaa agaaatgaaa
     3841 tttggattgt tcttccttaa cttcatcaat tcaacaactg ttcaagaaca aagtatagtt
     3901 cgcatgcagg aaataacgga gtatgttgat aagttgaatt ttgaacagat tttagtgtat
     3961 gaaaatcatt tttcagataa tggtgttgtc ggcgctcctc tgactgtttc tggttttctg
     4021 ctcggtttaa cagagaaaat taaaattggt tcattaaatc acatcattac aactcatcat
     4081 cctgtcgcca tagcggagga agcttgctta ttggatcagt taagtgaagg gagatttatt
     4141 ttagggttta gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg cccggttgaa
     4201 tatcaacagc aactatttga agagtgttat gaaatcatta acgatgcttt aacaacaggc
     4261 tattgtaatc cagataacga tttttatagc ttccctaaaa tatctgtaaa tccccatgct
     4321 tatacgccag gcggacctcg gaaatatgta acagcaacca gtcatcatat tgttgagtgg
     4381 gcggccaaaa aaggtattcc tctcatcttt aagtgggatg attctaatga tgttagatat
     4441 gaatatgctg aaagatataa agccgttgcg gataaatatg acgttgacct atcagagata
     4501 gaccatcagt taatgatatt agttaactat aacgaagata gtaataaagc taaacaagag
     4561 acgcgtgcat ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa tttcgaaaat
     4621 aaacttgaag aaataattgc agaaaacgct gtcggaaatt atacggagtg tataactgcg
     4681 gctaagttgg caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt tgaaccaatg
     4741 aatgatttga tgagccaaaa aaatgtaatc aatattgttg atgataatat taagaagtac
     4801 cacatggaat atacctaata gatttcgagt tgcagcgagg cggcaagtga acgaatcccc
     4861 aggagcatag ataactatgt gactggggtg agtgaaagca gccaacaaag cagcagcttg
     4921 aaagatgaag ggtataaaag agtatgacag cagtgctgcc atactttcta atattatctt
     4981 gaggagtaaa acaggtatga cttcatatgt tgataaacaa gaaattacag caagctcaga
     5041 aattgatgat ttgatttttt cgagcgatcc attagtgtgg tcttacgacg agcaggaaaa
     5101 aatcagaaag aaacttgtgc ttgatgcatt tcgtaatcat tataaacatt gtcgagaata
     5161 tcgtcactac tgtcaggcac acaaagtaga tgacaatatt acggaaattg atgacatacc
     5221 tgtattccca acatcggttt ttaagtttac tcgcttatta acttctcagg aaaacgagat
     5281 tgaaagttgg tttaccagta gcggcacgaa tggtttaaaa agtcaggtgg cgcgtgacag
     5341 attaagtatt gagagactct taggctctgt gagttatggc atgaaatatg ttggtagttg
     5401 gtttgatcat caaatagaat tagtcaattt gggaccagat agatttaatg ctcataatat
     5461 ttggtttaaa tatgttatga gtttggtgga attgttatat cctacgacat ttaccgtaac
     5521 agaagaacga atagattttg ttaaaacatt gaatagtctt gaacgaataa aaaatcaagg
     5581 gaaagatctt tgtcttattg gttcgccata ctttatttat ttactctgcc attatatgaa
     5641 agataaaaaa atctcatttt ctggagataa aagcctttat atcataaccg gaggcggctg
     5701 gaaaagttac gaaaaagaat ctctgaaacg tgatgatttc aatcatcttt tatttgatac
     5761 tttcaatctc agtgatatta gtcagatccg agatatattt aatcaagttg aactcaacac
     5821 ttgtttcttt gaggatgaaa tgcagcgtaa acatgttccg ccgtgggtat atgcgcgagc
     5881 gcttgatcct gaaacgttga aacctgtacc tgatggaacg ccggggttga tgagttatat
     5941 ggatgcgtca gcaaccagtt atccagcatt tattgttacc gatgatgtcg ggataattag
     6001 cagagaatat ggtaagtatc ccggcgtgct cgttgaaatt ttacgtcgcg tcaatacgag
     6061 gacgcagaaa gggtgtgctt taagcttaac cgaagcgttt gatagttgat atcctttgcc
     6121 tgcggccgca actagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     6181 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag
     6241 acctagcctt aataaggcct taattaaggc tagtaattat cattgactag cccatctcaa
     6301 ttggtatagt gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt
     6361 caaagcaaat gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat
     6421 tttatgctgt gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac
     6481 ggtatcgttc acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta
     6541 tggtgtatta gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc
     6601 tttggttaaa ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa
     6661 attagaatta gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat
     6721 aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga
     6781 gtggttatta aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct
     6841 tgatgaattt aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa
     6901 ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt
     6961 ggttgataag cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca
     7021 aatggatctc gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc
     7081 aacaaccatt acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc
     7141 tttaactgca aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag
     7201 taagcatgat ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga
     7261 gaacatactg gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg
     7321 aagcatcaag actaacaaac aaaagtagaa caactgttca ccgttagata tcaaagggaa
     7381 aactgtccat atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac
     7441 gagtttttgg tgcatttaaa gctgttcacc atgaacagat cgacaatgta acagatgaac
     7501 agcatgtaac acctaataga acaggtgaaa ccagtaaaac aaagcaacta gaacatgaaa
     7561 ttgaacacct gagacaactt gttacagctc aacagtcaca catagacagc ctgaaacagg
     7621 cgatgctgct tatcgaatca aagctgccga caacacggga gccagtgacg cctcccgtgg
     7681 ggaaaaaatc atggcaattc tggaagaaat agcgctttca gccggcaaac ctgaagccgg
     7741 atctgcgatt ctgataacaa actagcaaca ccagaacagc ccgtttgcgg gcagcaaaac
     7801 ccgtacccct taattaaggt ccttttcctt tgagttgtgg gtatctgtaa attctgctag
     7861 acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg
     7921 tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa
     7981 aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag
     8041 tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct
     8101 aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc
     8161 gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc
     8221 tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa
     8281 actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct
     8341 gctatgtggt gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt
     8401 ctgaccactt cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg
     8461 cagcggtatc atcaacaggc ttacccgtct tactgtccct agtgcttgga ttctcaccaa
     8521 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     8581 ttactggatc tatcaacagg agtccaagcg agctctcgaa ccccagagtc ccgctcagaa
     8641 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     8701 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     8761 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     8821 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     8881 atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc
     8941 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     9001 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     9061 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     9121 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     9181 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     9241 ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     9301 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     9361 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     9421 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca
     9481 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     9541 agagggcgcc ccagctggca attccgacgt ctaagaaacc attattatca tgacattaac
     9601 ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgag
//
