LOCUS       sequence-68294-e         929 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  To express ATPase inactive Fun30 in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Neves-Costa A, Will WR, Vetter AT, Miller JR, Varga-Weisz P
  TITLE     The SNF2-family member Fun30 promotes gene silencing in 
            heterochromatic loci.
  JOURNAL   PLoS One. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111.
  PUBMED    19956593
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-1"; volume: "4(12):e8111. doi"; pages: " 
            10.1371/journal.pone.0008111"
COMMENT     Sequence Label: pFA3
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(45..86)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cggcctctag aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg
       61 aggagagggt tagggatagg cttaccttcg aatgggtgac ctcgaagctc gcccttctcg
      121 agttctttgg ttcccttcgg tttcgagttt tcatcataaa ttatatcctc caacatatca
      181 ctaactttac tttccaacac atcttgagat tttttatctt cactgatata cgaatctaaa
      241 gctagtttat ttttggccag ttgatgaatc ttttcctcta tggaatcctt agtaattaaa
      301 gtggttatat taacttcctt tgtttgtccc acacgatgtg ccctatcagc agcttgtctg
      361 tcatcatgtg ggttaaaact ttgatcgaat ataataacat tatttgcgca caccaaatta
      421 ataccgaatc cacctgcctt tgttgataag atgaaaatgg gaatatcctt atcttcataa
      481 aacttatcta ttagtagttg tctatcattc acttgcgtgg aaccatctaa tcttaaaaat
      541 ttataatcta aggtggacaa aaccatctct agaatatcca ggacttgagt gaataaggaa
      601 aatatcagca ccttttcctg tttgtcaaca atgattgttt tcagcaattt tttcaaagcg
      661 tctatcttcc cagattgcat ccactcgtca ttatgaagtt ggtatttgga taacgtgttc
      721 gggaaattgc agcatagttt gtgcaactca aaatccgtca tatagctcat atcttcctta
      781 atatactctt tgttaccgtt ttcagcataa gcaggttcat ccaatatggc atcactcatt
      841 ttagtgatga ttttatcatt atagatattt ctgaacaaaa gtggatgcag agaggccttt
      901 cgcaatgcca ttattaaatt tttggaact
//
