LOCUS       sequence-68431-e        1003 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  TOP Hupki-wt plasmid without selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX41
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..37
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        19..37
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    244..313
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctcatccc
       61 cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta tccgcacccg ccgacgccgt
      121 cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg agggcttccc
      181 ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc gacccgttca tcatgatgga
      241 ccagatgggt gaggtggagt acgcgcccgg ggagcccaag ggcacgccct ggcacccgca
      301 ccgcggcttc gagaccgtga cctacatcgt ctctagtgcg gccgcacggc attttgagtg
      361 ttagactgga aactttccac ttgataagag gtcccaagac ttagtacctg aagggtgaaa
      421 tattctccat ccagtggttt cttctttggc tggggagagg agctggtgtt gttgggcagt
      481 gctaggaaag aggcaaggaa aggtgataaa agtgaatctg aggcataact gcacccttgg
      541 cctcctccac cgcttcttgt cctgcttgct tacctcgctt agtgctccct gggggcagct
      601 cgtggtgagg ctcccctttc ttgcggagat tctcttcctc tgtgcgccgg tctctcccag
      661 gacaggcaca aacacgcacc tcaaagctgt tccgtcccag tagattacca ctactcagga
      721 taggaaaaga gaagcaagag gcagtaagga aatcaggtcc tacctgtccc atttaaaaaa
      781 ccagactcca tctactccca accacccttg tcctttctgg agcctaagct ccagctccag
      841 gtaggtggag gagaagccac aggttaagag gtcccaaagc cagagaaaag aaaactgagt
      901 gggagcagta aggagattcc ccgccgggga tgtgatgaga ggtggatggg tagtagtatg
      961 gaagaaatcg gtaagaggtg ggcccagggg tcagaggcaa gca
//
