LOCUS       sequence-68460-e        7554 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length P38 ectodomain 
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: P38-bio
FEATURES             Location/Qualifiers
     source          1..7554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2934..2989
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2988..3007)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5225..5243)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5311..5415
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5416..5484,5485..6276)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5634..5653)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6447..7035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6936..6955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7189..7206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7323..7344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7359..7364,7365..7382,7383..7389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7397..7413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7402..7424
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7421..7437
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7421..7437
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcccagg tggagaacaa gaaggtggac ttccggaccg
     1381 agaagggcaa gttcgtgccc ctgaacctgg tgcctggcga cgtggtggag tacagctgcc
     1441 cctacagcct gaacaacgac atccggaaca tgaacggcgt ggagcgggag cacttcgaca
     1501 acaagaagtt ctgcttcgac tacatcttcg tgggcagcaa gctgaccttc ctgaaagaat
     1561 acgtgcgggg cagctacaac gtggtgcaca aagaggaagg caacctgtac accagccagt
     1621 tcagcgtgcc ccctgtggtg ctgacccacc ggaacttcga ctgcttctgc tacatggaag
     1681 agaacaacgt ggtcgtgaag aaggtgctgc ggatccacat cagcaacggc gtgctgagaa
     1741 agatccccgg ctgcgacttc aacgccgact acaaagagag caccgccatc accaccttca
     1801 gcaacatggc ccccagacgc gtgaaagtgt gcgacgtgta ccccaagagc ggcgacttca
     1861 tcagcctgat gtgccccagc gactacagca tcaagcccga cggctgcttc agcaacgtgt
     1921 acgtgaagag ataccccaac gaggaagtga aagaagagga ccggttcaac ctgaaccgga
     1981 agtgggacgc cagcaagtac aatgtggtgt ccatcgagac agtgctgaag atgaacatga
     2041 tcacccaggg cgacaagtac agcatcttca gcaagctgcc cgacgtgaag gaccaggtgg
     2101 actttacctg tatctgccag agcaacgacg agcaggacaa cctgatgatg aacgtgtaca
     2161 tcaacaacgc cgcctacctg accaacaacg cccggtccat cggcgtgaac aagcacagct
     2221 tctccaacag cgagatcttc gagcggatcg agcgggagga aatcagcggc gcgccgtcga
     2281 cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc ccactcaacc
     2341 ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct ccttcttccc
     2401 agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag tctactagca
     2461 accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc caggtctccc
     2521 ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata ctgtatcagg
     2581 aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg acctgtgagg
     2641 tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat caggaggcca
     2701 gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg gtgtggcaat
     2761 gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt ttatccaaag
     2821 ggttgaattc cggatcactg catcatattc tggatgcaca gaaaatggtg tggaatcatc
     2881 gttaagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     2941 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     3001 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     3061 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     3121 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     3181 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     3241 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     3301 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     3361 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3421 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3481 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3541 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3601 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3661 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     3721 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     3781 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     3841 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     3901 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     3961 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     4021 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     4081 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     4141 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     4201 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     4261 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     4321 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     4381 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4441 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4501 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4561 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4621 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4681 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4741 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4801 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     4861 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     4921 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4981 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5041 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     5101 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     5161 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     5221 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5281 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5341 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5401 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5461 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5521 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5581 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5641 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     5701 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5761 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5821 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5881 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5941 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6001 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6061 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6121 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6181 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6241 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6301 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6361 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6421 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6481 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6541 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6601 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6661 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6721 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6781 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6841 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6901 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6961 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7021 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7081 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7141 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7201 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7261 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7321 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7381 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7441 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     7501 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tgga
//
